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Thermo Scientific™ EZ-Link™ Maleimide-PEG11-Biotin

Catalog No. pi21911
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25 mg
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Catalog No. Quantity
PI21911 25 mg
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Catalog No. PI21911 Supplier Thermo Scientific™ Supplier No. LSG21911

Description

Biotinylate reduced cysteines with this long, maleimide-activated, sulfhydryl-reactive biotinylation reagent with an 11-unit PEG spacer arm to enhance solubility and reach.

Thermo Scientific™ EZ-Link Maleimide-PEG11-Biotin is a long, maleimide-activated, sulfhydryl-reactive biotinylation reagent that includes an 11-unit polyethylene glycol spacer arm for increased water-solubility and reach.

Highlights:

  • Polyethylene glycol (PEG) spacer yields biotin-labeled proteins with greater solubility and less aggregation
  • Forms stable thioether bond at pH 6.5-7.5
  • PEG spacer improves aqueous solubility, allowing labeling to be performed under non-denaturing, physiological conditions
  • Extended spacer arm reduces steric hindrance in binding to avidin, streptavidin and Thermo Scientific NeutrAvidin Proteins

Specifications

Product Type Maleimide-PEG11-Biotin
Quantity 25 mg
Content And Storage Store desiccated at 4°C. Shipped at ambient temperature.
Product Line EZ-Link
Cell Permeability Cell-Impermeant
Chemical Reactivity Thiol
Reactive Moiety Maleimide
Solubility DMF (Dimethylformamide), DMSO (Dimethylsulfoxide), Water
Label or Dye Biotin
Spacer Extra-long, Pegylated
Label Type Biotin & Analogs
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Frequently Asked Questions (FAQs)

What is the advantage of using EZ-Link HPDP-Biotin over maleimide- or iodoacetyl-containing biotinylation reagents?

Both maleimide- and iodoacetyl-containing biotinylation reagents react with sulfhydryl (-SH) groups to form stable thioether bonds that are not cleavable. EZ-Link HPDP-Biotin on the other hand is a pyridyldithiol-biotin compound that reacts with -SH groups in near-neutral buffers to form reversible disulfide bonds. Because the disulfide group can be cleaved using DTT or other reducing agents, HPDP-Biotin is useful for labeling and affinity-purification applications that require recovery of the original, unmodified molecule.

For Research Use Only. Not for use in diagnostic procedures.