Thermo Scientific™ EZ-Link™ HPDP-Biotin, No-Weigh™ Format

HPDP-Biotin is a pyridyldithiol-biotin compound for labeling protein cysteines and other molecules that contain sulfhydryl groups. This reagent specifically reacts with reduced thiols (-SH) in near-neutral buffers to form reversible disulfide bonds. HPDP-Biotin is useful for labeling and affinity-purification applications that require recovery of the original, unmodified molecule. For example, a protein can be biotinylated, allowed to bind its interactor, then captured to a streptavidin column and finally eluted and recovered by reduction of the disulfide bond with dithiothreitol.

• Protein labeling: Biotinylate antibodies or other proteins for use in protein methods
• Thiol-reactive: Reacts with sulfhydryls (-SH), such as the side-chain of cysteine (C)
• Pyridyldithiol-activated: Perform reactions at pH 6.5 to 7.5 in buffers such as PBS
• Reversible: Forms disulfide bonds, which can be cleaved using DTT or other reducing agent
• Solubility: Must be dissolved in DMSO or DMF before further dilution in aqueous buffers
• Medium length: Spacer arm (total length added to target) is 29.2A⁰

Thermo Scientific No-Weigh products are specialty reagents provided in a pre-aliquoted format. The pre-weighed packaging helps prevent the loss of reagent reactivity and contamination over time by minimizing the repetitive opening and closing of the vial. The format enables use of a fresh vial of reagent each time, eliminating the hassle of weighing small amounts of reagents and reducing concerns over reagent stability.