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  6. Thermo Scientific™ EZ-Link™ HPDP-Biotin, No-Weigh™ Format

Thermo Scientific™ EZ-Link™ HPDP-Biotin, No-Weigh™ Format

Catalog No. pia35390
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50 mg
10 x 1 mg
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Catalog No. Quantity
PIA35390 10 x 1 mg
PI21341 50 mg
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Catalog No. PIA35390 Supplier Thermo Scientific™ Supplier No. A35390
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Description

Thermo Scientific™ EZ-Link HPDP-Biotin is pyridyldithiol-activated, sulfhydryl-reactive biotinylation reagent that conjugates via a cleavable (reversible) disulfide bond to enable use in a variety of purification methods.

HPDP-Biotin is a pyridyldithiol-biotin compound for labeling protein cysteines and other molecules that contain sulfhydryl groups. This reagent specifically reacts with reduced thiols (-SH) in near-neutral buffers to form reversible disulfide bonds. HPDP-Biotin is useful for labeling and affinity-purification applications that require recovery of the original, unmodified molecule. For example, a protein can be biotinylated, allowed to bind its interactor, then captured to a streptavidin column and finally eluted and recovered by reduction of the disulfide bond with dithiothreitol.

  • Protein labeling: Biotinylate antibodies or other proteins for use in protein methods
  • Thiol-reactive: Reacts with sulfhydryls (-SH), such as the side-chain of cysteine (C)
  • Pyridyldithiol-activated: Perform reactions at pH 6.5 to 7.5 in buffers such as PBS
  • Reversible: Forms disulfide bonds, which can be cleaved using DTT or other reducing agent
  • Solubility: Must be dissolved in DMSO or DMF before further dilution in aqueous buffers
  • Medium length: Spacer arm (total length added to target) is 29.2A0

    • Thermo Scientific No-Weigh products are specialty reagents provided in a pre-aliquoted format. The pre-weighed packaging helps prevent the loss of reagent reactivity and contamination over time by minimizing the repetitive opening and closing of the vial. The format enables use of a fresh vial of reagent each time, eliminating the hassle of weighing small amounts of reagents and reducing concerns over reagent stability.

Specifications

Quantity 10 x 1 mg
Content And Storage Store in refrigerator (2–8°C).
Shipping Condition Room Temperature
Product Line EZ-Link, No-Weigh
Solubility DMF (Dimethylformamide), DMSO (Dimethylsulfoxide)
Format Solid
Label Type Biotin & Analogs

Frequently Asked Questions (FAQs)

What is the advantage of using EZ-Link HPDP-Biotin over maleimide- or iodoacetyl-containing biotinylation reagents?

Both maleimide- and iodoacetyl-containing biotinylation reagents react with sulfhydryl (-SH) groups to form stable thioether bonds that are not cleavable. EZ-Link HPDP-Biotin on the other hand is a pyridyldithiol-biotin compound that reacts with -SH groups in near-neutral buffers to form reversible disulfide bonds. Because the disulfide group can be cleaved using DTT or other reducing agents, HPDP-Biotin is useful for labeling and affinity-purification applications that require recovery of the original, unmodified molecule.
Is it possible that HPDP-biotin (Cat. No. 21341) can also react with non-reduced sulfur?

We have no evidence that HPDP-biotin (or any pyridyldithiol compound) reacts with anything other than sulfhydryl (-SH) groups. However, many sulfur-containing compounds interconvert among several different forms. For thiouracil, with the different tautomers that are possible, the =S is in equilibrium with –SH, which can then react with the HPDP.

For Research Use Only. Not for use in diagnostic procedures.