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Thermo Scientific™ EZ-Link™ HPDP-Biotin, No-Weigh™ Format

Catalog No. PI21341
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50 mg
10 x 1 mg
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Catalog No. Quantity
PI21341 50 mg
PIA35390 10 x 1 mg
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Catalog No. PI21341 Supplier Thermo Scientific™ Supplier No. 21341
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Description

Thermo Scientific™ EZ-Link HPDP-Biotin is pyridyldithiol-activated, sulfhydryl-reactive biotinylation reagent that conjugates via a cleavable (reversible) disulfide bond to enable use in a variety of purification methods.

Highlights:

  • Pyridyldithiol reacts with -SH groups to form a stable disulfide bond
  • Absorbance of pyridine-2-thione leaving group at 343nm can be used to determine reaction progress
  • Disulfide bond is cleavable using 50mM DTT (Part No. 20291) or 100mM β-Mercaptoethanol (Part No. 35600)
  • Extended spacer arm prevents steric hindrance
  • Must be dissolved in DMSO or DMF before use
  • Biotinylation occurs between pH 6-9

Specifications

Product Type HPDP-Biotin
Quantity 50 mg
Content And Storage Store at 4°C.
Product Line EZ-Link
Cell Permeability Cell-Permeant
Chemical Reactivity Thiol
Reactive Moiety Pyridyldisulfide
Solubility DMF (Dimethylformamide), DMSO (Dimethylsulfoxide)
Label or Dye Biotin
Spacer Long, Cleavable
Label Type Biotin & Analogs
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Frequently Asked Questions (FAQs)

What is the advantage of using EZ-Link HPDP-Biotin over maleimide- or iodoacetyl-containing biotinylation reagents?

Both maleimide- and iodoacetyl-containing biotinylation reagents react with sulfhydryl (-SH) groups to form stable thioether bonds that are not cleavable. EZ-Link HPDP-Biotin on the other hand is a pyridyldithiol-biotin compound that reacts with -SH groups in near-neutral buffers to form reversible disulfide bonds. Because the disulfide group can be cleaved using DTT or other reducing agents, HPDP-Biotin is useful for labeling and affinity-purification applications that require recovery of the original, unmodified molecule.
Is it possible that HPDP-biotin (Cat. No. 21341) can also react with non-reduced sulfur?

We have no evidence that HPDP-biotin (or any pyridyldithiol compound) reacts with anything other than sulfhydryl (-SH) groups. However, many sulfur-containing compounds interconvert among several different forms. For thiouracil, with the different tautomers that are possible, the =S is in equilibrium with –SH, which can then react with the HPDP.

For Research Use Only. Not for use in diagnostic procedures.