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Gibco™ ExpiFectamine™ Sf Transfection Reagent

Catalog No. A38915
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A38915 1 mL
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Catalog No. A38915 Supplier Gibco™ Supplier No. LSA38915
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Description

Gibco™ ExpiSf™ Transfection Reagant is a cationic lipid-based reagent for efficient DNA transfection of insect cells using fast, flexible protocols.

  • Delivers robust and reproducible transfection, giving you greater confidence in your results
  • Enables transfection of plasmid DNA in adherent and suspension Sf9 and Sf21 cell cultures for reliable recombinant baculovirus production
  • Facilitates efficient production of high-titer, high-quality P0 recombinant baculovirus in suspension culture format without the need for further virus amplification
  • Exhibits low toxicity and does not require medium change post-transfection
  • Is part of a simple, fast protocol with a short 5-minutes complexation time
  • Demonstrates linear scalability of transfections from culture volumes of 4mL to 500mL in suspension format

The ExpiFectamine Sf Transfection Reagent is a core component of the ExpiSf Expression System and has been optimized for transfection of ExpiSf9™ cells (Cat. No. A35243). However, the reagent also demonstrates superior performance using other Sf9 and Sf21 insect cells cultured in serum-free media such as Sf-900 II SFM or Sf-900 III SFM in both adherent and suspension culture formats.

Specifications

Content And Storage Store at 2–8°C, do not freeze

Shelf Life: 24 months from date of manufacture
Cell Type Insect Cells
Sample Type Baculovirus DNA, Plasmid DNA
Transfection Technique Lipid-based Transfection
For Use With (Application) Transfection
High-throughput Compatibility High-throughput Compatible
Product Line ExpiFectamine
Product Type Transfection Reagent
Quantity 1 mL
Serum Compatible Yes
Shipping Condition Approved for shipment at Room Temperature or on Wet Ice
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For Research Use Only. Not for use in diagnostic procedures.