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Gibco™ ExpiFectamine™ Sf Transfection Reagent 
Gibco™ ExpiSf™ Transfection Reagant is a cationic lipid-based reagent for efficient DNA transfection of insect cells using fast, flexible protocols.
Supplier: Gibco™ LSA38915
Description
- Delivers robust and reproducible transfection, giving you greater confidence in your results
- Enables transfection of plasmid DNA in adherent and suspension Sf9 and Sf21 cell cultures for reliable recombinant baculovirus production
- Facilitates efficient production of high-titer, high-quality P0 recombinant baculovirus in suspension culture format without the need for further virus amplification
- Exhibits low toxicity and does not require medium change post-transfection
- Is part of a simple, fast protocol with a short 5-minutes complexation time
- Demonstrates linear scalability of transfections from culture volumes of 4mL to 500mL in suspension format
The ExpiFectamine Sf Transfection Reagent is a core component of the ExpiSf Expression System and has been optimized for transfection of ExpiSf9™ cells (Cat. No. A35243). However, the reagent also demonstrates superior performance using other Sf9 and Sf21 insect cells cultured in serum-free media such as Sf-900 II SFM or Sf-900 III SFM in both adherent and suspension culture formats.
Specifications
| Store at 2–8°C, do not freeze Shelf Life: 24 months from date of manufacture |
|
| Baculovirus DNA, Plasmid DNA | |
| Transfection | |
| ExpiFectamine™ | |
| 1 mL | |
| Approved for shipment at Room Temperature or on Wet Ice |
| Insect Cells | |
| Lipid-based Transfection | |
| High-throughput Compatible | |
| Transfection Reagent | |
| Yes |
For Research Use Only. Not for use in diagnostic procedures.