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Gibco™ ExpiFectamine™ CHO Transfection Kit

Catalog No. p-6579024
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For 1 L of culture
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Catalog No. Quantity
A29129 For 1 L of culture
A29130 For 10 L of culture
A29131 For 50 L of culture
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Catalog No. A29129 Supplier Gibco™ Supplier No. A29129
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Description

The ExpiFectamine CHO Transfection Kit is a core component of the ExpiCHO Expression System, designed for transient transfection of high-density cultures of Chinese hamster ovary (CHO) cells.

The ExpiFectamine CHO Transfection Kit is a core component of the ExpiCHO Expression System Kit (Cat. No. A29133) and enables transient transfection of high-density cultures of Chinese hamster ovary (CHO) cells. The high-efficiency, cationic transfection reagent, coupled with an optimized transfection enhancer and culture feed, will help power the highest possible level of protein expression from ExpiCHO-S Cells (Cat. No. A29127) cultured in ExpiCHO Expression Medium (Cat. No. A2910001).

All components work together synergistically with expression yields of 1–3 g per L of transfected culture demonstrated for some antibody and non-antibody proteins.

Features of the ExpiFectamine CHO Transfection Kit include:

  • Enables robust and reproducible transfection results, giving you greater confidence in your results and ability to use CHO cells from research to discovery
  • Achieve >90% transfection efficiency of high-density suspension CHO cell culture, with matching transfection enhancer and culture feed to boost transfection performance and protein expression
  • Uses one-third to one-half less plasmid DNA than other transfection reagents
  • Scale growth and transfection in culture formats from <1 mL in deep well plates up to 5 L when using Nalgene angled-bottom shake flasks
  • Flexible, reliable culture and transfection protocols with multiple options that readily fit into current transient expression workflows

The ExpiFectamine CHO Transfection Kit is specifically designed to work with ExpiCHO-S Cells in the ExpiCHO Expression System Kit. The transfection kit supplies the cationic ExpiFectamine CHO Transfection Reagent, ExpiFectamine CHO Transfection Enhancer, and ExpiCHO Feed. ExpiCHO-S Cells and ExpiCHO Expression Medium are available separately.

Specifications

Cell Line ExpiCHO-S™, CHO-S, SFM Adapted
Classification Animal Origin-free, Chemically-defined, Protein-free, Serum-free
Content And Storage Kit includes:
• ExpiFectamine™ CHO Reagent, 3.2 mL
• ExpiFectamine™ CHO Enhancer, 6 mL
• ExpiCHO Feed, 330 mL

Sufficient for transfection of 1 L of culture
Store in the dark at 2°C to 8°C.
Cell Type Ovarian Cells
Format Kit
Promoter pcDNA™ 3.4
Transfection Technique Lipid-based Transfection
Product Line ExpiFectamine
Product Type CHO Transfection Kit
Quantity For 1 L of culture
Shipping Condition Room Temperature
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For Research Use Only. Not for use in diagnostic procedures.