MilliporeSigma™ pETcoco-2 DNA Expression Vector

Description

The pETcoco™-2 expression vector is designed to allow on command amplification of vector copy number from single copy (1–2 plasmid copies per cell) to medium copy (20–50 per cell). The vector is maintained in the single copy state by utiliziing the oriS and repE elements of the F episome together with the parABC partition determinants. The medium copy state is obtained by employing the RK-2 derived trfA replicator acting at oriV.