MilliporeSigma™ pET-43.1b(+) DNA Vector

The pET-43.1 series of vectors are designed for cloning and high-level expression of peptide sequences fused with the 491 aa Nus·Tag™ protein, the sequence is numbered by the pBR322 convention so the T7 expression region is reversed on the vector map, TB288VM. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand. Vector encoded sequence can be completely removed when cloning into the PshA I or Sma I sites by cleaving the Nus·Tag fusion protein with Enterokinase or Thrombin, respectively.