MilliporeSigma™ pET-41a(+) DNA Vector

Description

The pET-41 series is designed for cloning and high-level expression of peptide sequences fused with the 220 aa GST·Tag™ protein. The sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand. Vector encoded sequence can be completely removed when cloning into the PshAI site (as shown below) and then cleaving the GST fusion protein with Enterokinase.