MilliporeSigma™ pET-32 Xa/LIC Vector Kit

Description

The pET-32 Xa/LIC vector is an Xa/LIC version of pET-32b(+). It is designed for cloning and high-level expression of target proteins fused with the 109aa Trx·Tag™ thioredoxin protein, and His·Tag™ and S™ Tag™ coding sequences that are cleavable with Factor Xa protease. The plasmid contains a strong T7lac promoter, an optimized RBS, the coding sequence for the Xa protease cleavage site, and a multiple cloning site that contains restriction enzyme sites found in many other Novagen expression vectors to facilitate insert transfer.