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EMD Millipore Novagen™ Rosetta™ 2 (DE3) Competent Cells

$139.00 - $245.00

Specifications

Storage Requirements Store at ≤-70deg.C
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Products
Catalog Number Mfr. No. Quantity Price Quantity    

713973

 
emd millipore corporation
71397-3
0.4mL Each for $139.00

713974

 
emd millipore corporation
71397-4
1mL Each for $245.00
Description & Specifications

Specifications

Storage Requirements Store at ≤-70deg.C

Rosetta™ 2 host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. These strains supply tRNAs for 7 rare codones (AGA, AGG, AUA, CUA, GGA, CCC, and CGG) on a compatible chloramphenicol-resistant plasmid. The tRNA genes are driven by their native promoters. DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.