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EMD Millipore Novagen™ Rosetta™ 2 (DE3) Competent Cells

$139.00 - $245.00


Storage Requirements Store at ≤-70deg.C
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Catalog Number Mfr. No. Quantity Price Quantity    


emd millipore corporation
0.4mL Each for $139.00


emd millipore corporation
1mL Each for $245.00
Description & Specifications


Storage Requirements Store at ≤-70deg.C

Rosetta™ 2 host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. These strains supply tRNAs for 7 rare codones (AGA, AGG, AUA, CUA, GGA, CCC, and CGG) on a compatible chloramphenicol-resistant plasmid. The tRNA genes are driven by their native promoters. DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.