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MilliporeSigma™ Upstate™ Monomethyl-Histone H4 (Lys20), Rabbbit Polyclonal, ChIP Validated Antibody and Primer Set
Rabbit Polyclonal Antibody
Supplier: MilliporeSigma™ 17651
Description
Specifically detects Monomethyl-Histone H4 (Lys20) Clone: in Human, Plant, Vertebrate samples, and it is validated for Chromatin Immunoprecipitation, Western Blotting
Specifications
| Monomethyl-Histone H4 (Lys20) | |
| Polyclonal | |
| Anti-monomethyl-Histone H4 (Lys20) (rabbit polyclonal IgG). One vial containing 100μg of affinity purified antibody in 100μL 0.1M Tris-Glycine (pH 7.4), 15mM NaCl, and 0.05% NaN3. Normal Rabbit IgG. One vial containing 125μg of purified rabbit IgG in 125μL storage buffer. ChIP Primers GAPDH Coding Region. One vial containing 75μL of 5μM of each primer specific for human GAPDH coding region. FOR: GGC TCC CAC CTT TCT CAT CC; REV: GGC CAT CCA CAG TCT TCT GG | |
| H4/A , H4/B, H4/C , H4/D, H4/E, H4/G, H4/H, H4/I, H4/J, H4/J, H4/M, H4/N, H4/O , H4/a , H4F2, H4FA , H4FB , H4FC , H4FD, H4FE, H4FG, H4FH, H4FI , H4FJ, H4FK, H4FM , H4FN, H4FO, HIST2H4 | |
| The immunogen was a synthetic peptide corresponding to amino acids 15-24 of histone H4, monomethylated on Lys20. | |
| 25 Assays | |
| Epigenetics and Nuclear Function | |
| NM_003538.3 | |
| This ChIPAb+ Monomethyl-Histone H4 (Lys20) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers. | |
| -20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles |
| ChIP Assay, Western Blot | |
| Unconjugated | |
| P62805 | |
| Rabbit | |
| Affinity Purified | |
| RUO | |
| Primary | |
| Recognizes histone H4, Mr ∽11kDa. | |
| Human, Plant, Vertebrates |
For Research Use Only