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MilliporeSigma™ Upstate™ Monomethyl-Histone H4 (Lys20), Rabbbit Polyclonal, ChIP Validated Antibody and Primer Set

Catalog No. 17651MI
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Quantity:
25 Assays
Conjugate:
Unconjugated
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Catalog No. Quantity Conjugate
17651MI 25 Assays Unconjugated
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Catalog No. 17651MI Supplier MilliporeSigma™ Supplier No. 17651
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Description

Rabbit Polyclonal Antibody

Specifically detects Monomethyl-Histone H4 (Lys20) Clone: in Human, Plant, Vertebrate samples, and it is validated for Chromatin Immunoprecipitation, Western Blotting

Specifications

Antigen Monomethyl-Histone H4 (Lys20)
Applications ChIP Assay, Western Blot
Classification Polyclonal
Conjugate Unconjugated
Formulation Anti-monomethyl-Histone H4 (Lys20) (rabbit polyclonal IgG). One vial containing 100μg of affinity purified antibody in 100μL 0.1M Tris-Glycine (pH 7.4), 15mM NaCl, and 0.05% NaN3. Normal Rabbit IgG. One vial containing 125μg of purified rabbit IgG in 125μL storage buffer. ChIP Primers GAPDH Coding Region. One vial containing 75μL of 5μM of each primer specific for human GAPDH coding region. FOR: GGC TCC CAC CTT TCT CAT CC; REV: GGC CAT CCA CAG TCT TCT GG
Gene Accession No. P62805
Gene Symbols H4/A , H4/B, H4/C , H4/D, H4/E, H4/G, H4/H, H4/I, H4/J, H4/J, H4/M, H4/N, H4/O , H4/a , H4F2, H4FA , H4FB , H4FC , H4FD, H4FE, H4FG, H4FH, H4FI , H4FJ, H4FK, H4FM , H4FN, H4FO, HIST2H4
Host Species Rabbit
Immunogen The immunogen was a synthetic peptide corresponding to amino acids 15-24 of histone H4, monomethylated on Lys20.
Purification Method Affinity Purified
Quantity 25 Assays
Regulatory Status RUO
Research Discipline Epigenetics and Nuclear Function
Primary or Secondary Primary
Gene ID (Entrez) NM_003538.3
Test Specificity Recognizes histone H4, Mr ∽11kDa.
Includes This ChIPAb+ Monomethyl-Histone H4 (Lys20) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Target Species Human, Plant, Vertebrates
Content And Storage -20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
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For Research Use Only