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MilliporeSigma™ Upstate™ E2F-1α, Mouse monoclonal, ChIP Validated Antibody and Primer Set

Catalog No. 1710061MI
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Quantity:
25 Assays
Conjugate:
Unconjugated
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Catalog No. Quantity Conjugate
1710061MI 25 Assays Unconjugated
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Catalog No. 1710061MI Supplier MilliporeSigma™ Supplier No. 1710061
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Description

Mouse Monoclonal Antibody

Specifically detects E2F-1α Clone: in Human, Xenopus samples, and it is validated for ChIP, ChIP-seq, Immunoprecipitation

E2F-1 was identified as a cellular protein with DNA binding activity associated with the adenovirus E2 gene promoter. The original member of the E2F family of transcription factors, now consisting of five, was identified through its association with the retinoblastoma protein, which controls its activity. E2F-1 is cell cycle regulated with very low levels in early G1 then increasing levels as cells move from G1 to S with highest levels of protein at the G1/S boundary, which is consistent with its role in S-phase entry.

Specifications

Antigen E2F-1α
Applications ChIP Assay, ChIP sequencing (ChIP-seq), Immunoprecipitation
Classification Monoclonal
Conjugate Unconjugated
Formulation Anti-E2F-1 (mouse monoclonal). One vial containing 25μg of protein G purified monoclonal mixed IgG in 25μL of buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl with 0.05% sodium azide. Frozen solution. Nornal Mouse IgG. One vial containing 25μg of purified IgG in 25μL of storage buffer containing 0.1% sodium azide. ChIP Primers, CDC2 promoter. One vial containing 75μL of 5μM each primer specific for human CDC2 promoter. FOR: CGC CCT TTC CTC TTT CTT TC; REV: ATC GGG TAG CCC GTA GAC TT
Gene Accession No. Q01094
Gene Symbols E2F1, RBAP-1, E2F-1, RBP3, RBBP3, RBBP-3, PBR3
Host Species Mouse
Immunogen GST fusion protein corresponding to full-length human E2F-1.
Purification Method Protein G purified
Quantity 25 Assays
Regulatory Status RUO
Research Discipline Epigenetics and Nuclear Function
Primary or Secondary Primary
Gene ID (Entrez) NP_005216
Test Specificity The epitopes have been mapped to amino acids 1 to 89 and to amino acids 342 to 386.
Includes This ChIPAb+ E2F-1 -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Target Species Human, Xenopus species
Content And Storage -20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Form Purified
Isotype IgG
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For Research Use Only