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MilliporeSigma™ Upstate™ Acetyl-Histone H4 (Lys5)α, Rabbit Monoclonal, ChIP Validated Antibody and Primer Set

Catalog No. 1710045MI
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Quantity:
25 Assays
Conjugate:
Unconjugated
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Catalog No. Quantity Conjugate
1710045MI 25 Assays Unconjugated
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Catalog No. 1710045MI Supplier MilliporeSigma™ Supplier No. 1710045
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Description

Rabbit Monoclonal Antibody

Specifically detects Acetyl-Histone H4 (Lys5)α Clone: in Human samples, and it is validated for Chromatin Immunoprecipitation, Immunoprecipitation, Western Blotting

Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).

Specifications

Antigen Acetyl-Histone H4 (Lys5)α
Applications ChIP Assay, Immunoprecipitation, Western Blot
Classification Monoclonal
Conjugate Unconjugated
Formulation Anti-Acetyl-Histone H4 (Lys5) (rabbit monoclonal IgG). One vial containing 50μL of rabbit monoclonal IgG cell culture supernatant in 0.1% sodium azide. Negative Control Supernatant (rabbit). One vial containing 50μL of cultured supernatant in 0.05% sodium azide. Control Primers. One vial containing 75μL of 5μM each primer specific for the human GAPDH promoter. FOR: TAC TAG CGG TTT TAC GGG CG; REV: TCG AAC AGG AGG AGC AGA GAG CGA
Gene Accession No. P62805
Gene Symbols HIST1H4A, H4/J, HIST1H4F, HIST1H4L, H4FN, H4FA, H4FH, HIST1H4H, H4F2, H4FM, H4/A, H4FK, H4FG, HIST2H4, H4FB, H4/K, HIST1H4I, H4/N, HIST1H4B, H4FE, H4/M, H4/a, HIST1H4E, HIST4H4, HIST2H4A, H4FC, H4FI, H4/H, HIST1H4C, H4/G, HIST1H4K, H4FJ, H4FD, H4/I, H4/B, H4/D, H4/C, HIST1H4J, HIST1H4D, H4/E
Host Species Rabbit
Immunogen Peptide corresponding to Histone H4 containing the sequence [GRG-AcK-GGK] on which Lys5 is acetylated.
Quantity 25 Assays
Regulatory Status RUO
Research Discipline Epigenetics, Nuclear Function
Primary or Secondary Primary
Gene ID (Entrez) NP_001029249
Test Specificity Recognizes Histone H4 acetylated on Lys5.
Includes This ChIPAb+ Acetyl-Histone H4 (Lys5) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Target Species Human
Content And Storage -20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Form Culture Supernatant
Isotype IgG
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For Research Use Only