Invitrogen™ EGFR Monoclonal Antibody (H11)

Description

MA1-12693 detects Epidermal Growth Factor Receptor in human samples. MA1-12693 has been successfully used in FACS, immunofluorescence, immunoprecipitation, Western blot, and immunohistochemistry (paraffin) procedures. Staining of formalin-fixed tissues requires digestion with protease xxv at 1 mg/mL in PBS for 5 minutes at 37°C. Positive controls include A431 cells or placenta or squamous cell carcinoma. Suitable for Western blotting of extracellular domain of EGFR. The MA1-12693 immunogen is HC2 20 d2 cells.

EGFR, epidermal growth factor receptor, is a receptor tyrosine kinases that signals in response to various growth factors. Overexpression has been linked to numerous types of cancer and EGFR is the target of both biological and small molecular therapeutics. EGFR is encoded by the EGFR gene located on chromosome 7 in humans. EGFR belongs to the HER/ERbB family of proteins that includes three other receptor tyrosine kinases, ERbB2, ERbB3, ERbB4. EGFR is a transmembrane receptor and binding of its cognate ligands such as EGF (Epidermal Growth Factor) and TGF alpha (Transforming Growth Factor alpha) to the extracellular domain leads to EGFR dimerization followed by autophosphorylation of the tyrosine residues in the cytoplasmic domain. Overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.