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Invitrogen™ Donkey anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, FITC

Catalog No. PI31568
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500 μg
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Catalog No. Quantity
PI31568 500 μg
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Catalog No. PI31568 Supplier Invitrogen™ Supplier No. 31568
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Description

Donkey Polyclonal Secondary Antibody

Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration. Product # 31568 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications. Product # 31568 reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins, but does not react against non-immunoglobulin serum proteins. This antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, chicken, goat, guinea pig, hamster, horse, human, mouse,rat and sheep serum proteins. However, this antibody may cross-react with immunoglobulins from other species. Store product protected from light at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C. Fluorescein Amax= 492 nm; Emax= 520 nm. Fluorophore/Protein: 8.3 μg/mg; >3 moles FITC per mole IgG. (lot-dependent). Reconstitute with 0.4 mL of distilled water (1.5 mg/mL after restoration). Country of Origin: USA.

Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

Antigen Rabbit IgG (H+L) Cross-Adsorbed
Applications Immunohistochemistry, Immunoprecipitation, Immunocytochemistry
Classification Polyclonal
Concentration 1.5 mg/mL
Conjugate FITC
Formulation PBS with 15mg/mL BSA and 0.05% sodium azide; pH 7.6
Host Species Donkey
Purification Method Antigen affinity chromatography
Quantity 500 μg
Regulatory Status RUO
Primary or Secondary Secondary
Target Species Rabbit
Content And Storage 4°C, store in dark
Product Type Secondary Antibody
Form Lyophilized
Isotype IgG
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