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Invitrogen™ DNA Retardation Gels (6%), 1.0 mm
Description
Includes
Catalog numbers for gels refer to a single box. One box contains 10 gels.
Novex™ DNA Retardation Gels are 6% polyacrylamide prepared with 0.5X TBE gel buffer, providing clear resolution of fragments used for DNA retardation assays from 60–2500 bp. This preparation is good for electrophoretic separation, yet low enough to promote DNA-protein interactions. Novex DNA Retardation Gels are designed to run on the XCell SureLock™ Mini-Cell and are available in 10-, 12-, or 15-well formats.
• DNA fragments ranging from 60-2,500 bp
• Prepared with 0.5X TBE as gel buffer
• Designed to run on the XCell SureLock Mini-Cell
• Available in 10, 12, and 15 well formats
• Manufactured with high-purity tris base, boric acid, EDTA, acrylamide, bisacrylamide, TEMED, and APS
To ensure best possible results, these gels are single use and individually wrapped. Detection is performed with ethidium bromide staining of DNA or, for greater sensitivity, with radiolabeling the DNA or protein.
Formulation
Novex DNA Retardation Gels are manufactured with high-purity tris base, boric acid, EDTA, acrylamide, bisacrylamide, TEMED, and APS
Recommended buffers
For optimum performance, Novex™ TBE Running Buffer and Novex™ Hi-Density TBE Sample Buffer are strongly recommended for use with these gels. Novex Hi-Density TBE Sample Buffer contains the tracking dyes Bromophenol Blue and Xylene Cyanol as well as the density agent Ficoll™, which yields sharper and straighter bands than conventional density agents.
Order Info
Shipping Condition: Wet Ice
Specifications
Specifications
| Content And Storage | • Catalog number refers to a single box of 10 gels Store at 4°C. |
| Description | DNA Retardation Gels, 10-well |
| Gel Percentage | 6% |
| Gel Thickness | 1 mm |
| Gel Type | DNA Retardation |
| Separation Range | 60 to 2500 bp |
| Wells | 10-well |
| Quantity | 10 Gels/Box |
| Sample Buffer Type | TBE Hi-Density Sample Buffer (5X) |
| Sample Type | Double-stranded DNA (dsDNA) |
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Frequently Asked Questions (FAQs)
A shift assay is a DNA-binding assay using nondenaturing PAGE. It provides a simple, rapid, and extremely sensitive method for detecting sequence-specific DNA-binding proteins. Proteins bind specifically to an end-labeled DNA fragment corresponding to the individual protein-DNA complexes. You can use the assay to test binding of purified proteins or of uncharacterized factors in crude extracts. This assay also permits quantitative determination of the affinity, abundance, association rate constants, dissociation rate constants, and binding specificity of DNA-binding proteins.
A supershift assay is a variation of the mobility shift DNA-binding assay that uses antibodies to identify proteins present in the protein-DNA complex.Addition of a specific antibody to a binding reaction can have one of several effects. If the protein recognized by the antibody is not involved in complex formation, addition of the antibody should have no effect. If the protein that forms the complex is recognized by the antibody, the antibody can either block complex formation or it can form an antibody-protein-DNA ternary complex and thereby specifically result in a further reduction in the mobility of the protein-DNA complex (a supershift). Results may be different depending upon whether the antibody is added before or after the protein binds DNA (particularly if there are epitopes on the DNA binding surface of the protein).
The Invitrogen 6% DNA Retardation Gels contain 0.5X TBE. Both gels will work for gel retardation; however, the 1X TBE in the Invitrogen 6% TBE Gels have a higher ionic environment, which may affect DNA-protein interactions. The 0.5X TBE used in the Invitrogen 6% DNA Retardation Gels usually works better, as it offers good fragment separation in electrophoresis yet has an ionic strength low enough to promote DNA-protein interactions.
The 6% DNA retardation gels are 0.5X TBE. Both gels will work for gel mobility shift assays, but the 1X TBE has a higher ionic environment that may affect DNA/protein interactions. 0.5X TBE usually works better.