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Applied Biosystems™ dGTP BigDye™ Terminator v3.0 Ready Reaction Cycle Sequencing Kit

Catalog No. 4390229
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100 reactions
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4390229 100 reactions
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Catalog No. 4390229 Supplier Applied Biosystems™ Supplier No. 4390229
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Description

Includes

Contains the AmpliTaq™ DNA Polymerase, FS, BigDye terminators v3.0, pGEM™ - 3Zf(+) Control Template, -21 M13 Control Primer (forward), and Premixed Ready Reaction.

The dGTP BigDye™ terminator cycle sequencing kit's fluorescent dideoxy terminator chemistry is optimized for sequencing through GT- and G-rich templates.

  • Ensure reliable results with difficult regions and finishing applications
  • Sequence templates with challenging sequence contexts
  • Sequence problematic templates that standard chemistries cannot
  • The dGTP BigDye™ Terminator Kit replaces dITP with dGTP and it enables you to extend through those difficult-to-sequence regions with confidence, avoiding early signal loss in these samples
  • Optimized for GT-Rich templates: Obtain higher success rate even with your difficult GT-rich templates
  • By using the same chemistries as the standard BigDye™ Terminator Kits (only replacing dITP with dGTP) the dGTP BigDye™ Terminator Kit provides proven performance
  • Optimized for GT-rich and other difficult-to-sequence templates

Order Info

100 rxns

Specifications

Content And Storage The kit contains the AmpliTaq™ DNA Polymerase, FS, BigDye
terminators v3.0, pGEM™ - 3Zf(+) Control Template,
-21 M13 Control Primer (forward), and Premixed Ready Reaction.
Store kit at -15 to -25°C.
Format 12-well Strips, 8-well Strip, 96- or 384-well Plate
For Use With (Application) GT-Rich or Difficult Template Sequencing
No. of Reactions 100 reactions
Product Line BigDye Terminator, BigDye
Product Type Terminator V3.0 Ready Reaction Cycle Sequencing Kit
Quantity 100 reactions
Shipping Condition Dry Ice
Technique Fluorescent Dye Terminator Sequencing
Template Compatibility BAC DNA, Genomic DNA (Bacterial), Plasmid DNA (≤15Kb), PCR Amplicons, Single Stranded DNA

For Research Use Only. Not for use in diagnostic procedures.