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Applied Biosystems™ dGTP BigDye™ Terminator v1.0 Ready Reaction Cycle Sequencing Kit DFS Item

dGTP BigDye™ terminator cycle sequencing kit with the AmpliTaq™ DNA polymerase, FS. This ready reaction kit uses the same dye set as the BigDye™ v1.0 kits.

Supplier:  Applied Biosystems™ 4307175

Catalog No. 4307175


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Includes: Contains AmpliTaq™ DNA Polymerase, FS, BigDye terminators v1.0, pGEM™ - 3Zf(+) Control Template, -21 M13 Control Primer (forward), and Premixed Ready Reaction.

Description

Description

  • Ensure reliable results with difficult regions and finishing applications
  • Sequence templates with challenging sequence contexts
  • Sequence problematic templates that standard chemistries cannot
  • Optimized for GT-Rich templates: Obtain higher success rate even with your difficult GT-rich templates
  • By using the same chemistries as the standard BigDye™ terminator kits (only replacing dITP with dGTP) the dGTP BigDye™ terminator kit provides proven performance
  • Optimized for GT-rich and other difficult-to-sequence templates
  • Easily Integrate into your workflow: dGTP BigDye™ terminator kits require no new software or instrument recalibration, so you can easily integrate it into your current workflow with minimal changes to your protocols
  • The dGTP BigDye™ terminator kit replaces dITP with dGTP and it enables you to extend through those difficult-to-sequence regions with confidence, avoiding early signal loss in these samples

Order Info

100 rxns

Specifications

Specifications

The kit contains the AmpliTaq™ DNA Polymerase, FS, BigDye
terminators v1.0, pGEM™ - 3Zf(+) Control Template,
-21 M13 Control Primer (forward), and Premixed Ready Reaction.
Store kit at -15 to -25°C.
GT-Rich or Difficult Template Sequencing
BigDye™ Terminator, BigDye™
100 Reactions
BAC DNA, Genomic DNA (Bacterial), Plasmid DNA (≤15Kb), PCR Amplicons, Single Stranded DNA
12-well Strips, 8-well Strip, 96- or 384-well Plate
100 reactions
Sequencing Kit
Fluorescent Dye Terminator Sequencing
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For Research Use Only. Not for use in diagnostic procedures.