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Invitrogen™ Mouse Decorin (DCN) ELISA Kit
ELISA
$914.00
Specifications
| Accession Number | P28654 |
|---|---|
| Assay Range | 4.1 to 1000 pg/mL |
| Assay Sensitivity | 5 pg/mL |
| Calibration Range | 5 pg/mL to 1000 pg/mL |
| Assay | Solid-phase sandwich ELISA |
Description
The Mouse Decorin (DCN) ELISA quantitates Ms Decorin in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms Decorin. Principle of the method The Mouse Decorin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Decorin is a member of the small, leucine-rich proteoglycan (SLRP) family along with biglycan. Found in the interterritorial region of cartilage, decorin interacts with collagen and other matrix proteins to regulate the cartilaginous matrix. It has been shown to interact with fibronectin, thrombospondin, C1q, epidermal growth factor receptor (EGFR), and TGF-b. It is believed that decorin also plays a role in the cell cycle of chondrocytes. The decorin neoepitope fragment has a sequence of PKTLQE, and is generated by MMP-3 or ADAMTS-4 cleavage at Glu154-Leu155 of decorin core protein.Specifications
| P28654 | |
| 5 pg/mL | |
| Solid-phase sandwich ELISA | |
| ELISA | |
| Plasma, Serum, Supernatant | |
| Mouse | |
| Colorimetric Microplate Reader | |
| DC,DSPG2,PG40,PGII,PGS2,SLRR1B | |
| <10 | |
| HRP | |
| Development | |
| 2°C to 8°C | |
| Decorin | |
| 4 hrs 45 mins |
| 4.1 to 1000 pg/mL | |
| 5 pg/mL to 1000 pg/mL | |
| HRP | |
| Other Proteins | |
| This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP- 5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, PSelectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF RI, TNF RII, TPO, VCAM-1, VEGF. | |
| ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis | |
| 13179 | |
| <12% | |
| Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers | |
| RUO | |
| Plasma 1 μL, Serum 1 μL, Supernatant 100 μL | |
| DC, DSPG2, PG40, PGII, PGS2, SLRR1B, mDcn | |
| 1 hrs 20 mins |