CXCL5 Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Description

AA Sequence of recombinant protein: AAVLRELRCV CLQTTQGVHP KMISNLQVFA IGPQCSKVEV VASLKNGKEI CLDPEAPFLK KVIQKILDGG NKEN. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human ENA-78 (CXCL5). Anti-Human ENA-78 (CXCL5)-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect hENA-78 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human ENA-78 (500-P91) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hENA-78. Western Blot: To detect hENA-78 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hENA-78 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P91BT-1MG will be provided as 2 x 500 μg

The protein encoded by this gene is an inflammatory chemokine that belongs to the CXC chemokine family. This chemokine is produced concomitantly with interleukin-8 (IL8) in response to stimulation with either interleukin-1 (IL1) or tumor necrosis factor-alpha (TNFA). This chemokine is a potent chemotaxin involved in neutrophil activation.