Invitrogen™ IP-10/CXCL10 Mouse Matched Antibody Pair

Comprend

• 1 vial (0.275mL) polyclonal Coating Antibody to mouse IP-10 (100μg/mL)
• 1 vial (55μL) Biotin-Conjugate anti-mouse IP-10 polyclonal antibody
• 1 vial (22μL) Streptavidin-HRP
• 10 vials mouse IP-10 Standard protein lyophilized, 1ng/mL upon reconstitution
• 2 vials (50mL) Sample Diluent

Matched Antibody Pair

The Mouse IP-10 Matched Antibody Pair Kit comes with pre-matched antibody pairs,standards,sample dilutent and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse IP-10. Buffer reagents and microplates needed to complete the ELISA reaction are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate,which is an overnight process. Samples,standards,or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody,a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.