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Invitrogen™ Human Cu/ZnSOD Matched Antibody Pair

Catalog No. 5018056
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10 x 96 Tests
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Catalog No. Quantity
5018056 10 x 96 Tests
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Catalog No. 5018056 Supplier Invitrogen™ Supplier No. BMS222MST

Description

ELISA

The Human Cu/ZnSOD Matched Antibody Pair Kit comes with pre-matched antibody pairs and standards to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human Cu/ZnSOD. Buffer reagents and microplates needed to complete the ELISA reaction are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

SOD1 (superoxide dismutase) binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. This isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene.

Specifications

Accession Number P00441
Antigen Cu/ZnSOD
Assay Range 0.08-5 ng/mL
Applications ELISA
Assay Sensitivity 0.08 ng/mL
Classification Monoclonal
Conjugate HRP
Gene Accession No. P00441
Gene Alias ALS; als1; Amyotrophic lateral sclerosis 1 (ALS1); B430204E11Rik; Cu(2+)-Zn2+ superoxide dismutase; Cu/Zn SOD; Cu/Zn superoxide dismutase; Cu/Zn-SOD; CuZn superoxide dismutase; Cu-Zn superoxide dismutase; Cu-Zn superoxide dismutase C-terminal fragment (150AA); CuZnSOD; Cu-Zn-superoxide dismutase; epididymis secretory protein Li 44; HEL-S-44; HGNC:11179; homodimer; hSod1; indophenoloxidase A; Indophenoloxidase A (IPOA); Ipo1; Ipo-1; ipoa; similar to superoxide dismutase; sod; SOD, soluble; sod1; Sod-1; sod1.S; sod1-a; sod1-b; SOD1L1; SODC; superoxide dismutase; superoxide dismutase [Cu-Zn]; Superoxide dismutase [Cu-Zn] A; superoxide dismutase [Cu-Zn] A-like; superoxide dismutase 1; Superoxide Dismutase 1 (SOD1); superoxide dismutase 1 S homeolog; superoxide dismutase 1 soluble; superoxide dismutase 1, soluble; superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)); superoxide dismutase 1, soluble S homeolog; superoxide dismutase, cystolic; XELAEV_18014289mg; XSODA; xsodbALS; als1; Amyotrophic lateral sclerosis 1 (ALS1); B430204E11Rik; Cu(2+)-Zn2+ superoxide dismutase; Cu/Zn SOD; Cu/Zn superoxide dismutase; Cu/Zn-SOD; CuZn superoxide dismutase; Cu-Zn superoxide dismutase; Cu-Zn superoxide dismutase C-terminal fragment (150AA); CuZnSOD; Cu-Zn-superoxide dismutase; epididymis secretory protein Li 44; HEL-S-44; HGNC:11179; homodimer; hSod1; indophenoloxidase A; Indophenoloxidase A (IPOA); Ipo1; Ipo-1; ipoa; similar to superoxide dismutase; sod; SOD, soluble; sod1; Sod-1; sod1.S; sod1-a; sod1-b; SOD1L1; SODC; superoxide dismutase; superoxide dismutase [Cu-Zn]; Superoxide dismutase [Cu-Zn] A; superoxide dismutase [Cu-Zn] A-like; superoxide dismutase 1; Superoxide Dismutase 1 (SOD1); superoxide dismutase 1 S homeolog; superoxide dismutase 1 soluble; superoxide dismutase 1, soluble; superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)); superoxide dismutase 1, soluble S homeolog; superoxide dismutase, cystolic; XELAEV_18014289mg; XSODA; xsodb; ALS; ALS1; HEL-S-44; IPOA; SOD; hSod1; homodimer
Gene Symbols SOD1
Immunoassay Kit Format Matched Antibody Pair
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
Quantity 10 x 96 Tests
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 6647
Target Species Human
For Use With (Equipment) Absorbance Microplate Reader
Content And Storage 1 vial (2.75 mL) monoclonal Coating Antibody to human Cu/ZnSOD (100 ug/mL)
1 vial (55 uL) HRP-Conjugate anti-human Cu/ZnSOD monoclonal antibody
1 vial (60 uL) human Cu/ZnSOD Standard protein, 400 ng/mL
Gene Symbol SOD1
Kit Contents Capture (coating) Antibody, Detection Antibody, Standard
Label or Dye HRP
Protein Aliases Superoxide dismutase 1, hSod1
Storage Requirements -20°C
Target SOD1
Test Time 1 hr 10 min
Total Assay Time 24 hr
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