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MP Biomedicals™ CSM-His-Leu-Met-Trp-Ura (Powder)
Custom formulate your own selective Synthetic Defined yeast media. MP Biomedicals CSM-His-Leu-Met-Trp-Ura (Powder) is a 5 dropout of Complete Supplement Mixture of amino acids for S. cerevisiae growth media. For use with DOB or YNB with the appropriate ni
Supplier: MP Biomedicals™ 114550422
Description
5 Dropout Complete Supplement Mixture is used to select for yeast containing plasmids with specific nutritional markers and to select for gene function in transformation experiments. Reagents are tested and chosen for their ability to promote optimum growth of yeast strains when combined in the DOB or DOBA with this formula.
Chosen reagents are combined in the proper proportions according to the formulation listed in the certificate of analysis and milled to a powder which thoroughly blends the reagents for uniform distribution.
- A sample of the milled and blended formulation was used to prepare liquid plate medium 43.7g DOBA powder and 0.56g of CSM-His-Leu-Met-Trp-Ura were added to 1L of purified water
- The combinations were mixed by hand for about 1 minute to dissolve the dextrose
- The mixture was then autoclaved at 121°C for 25 minutes After cooling to 50°C, plates were poured
Specifications
| 5 Dropout Complete Supplement Mixture used to select for yeast containing plasmids with specific nutritional markers and to select for gene function in transformation experiments. | |
| 100 g |