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Invitrogenā¢ CellTraceā¢ Far Red Cell Proliferation Kit, for flow cytometry 
CellTraceā¢ Far Red Cell Proliferation Kit, for flow cytometry
Supplier: Invitrogenā¢ C34564
Description
CellTraceā¢ Far Red Cell Proliferation Kit is used for in vitro and in vivo labeling of cells to trace multiple generations using dye dilution by flow cytometry.View a selection guide for all CellTraceā¢ Cell Proliferation Kits for flow cytometry.
Superior fluorescent staining
Successful proliferation analysis by dye dilution requires an extremely bright dye to distinguish fluorescently labeled cells from auto-fluorescence after several cell divisions. The intense fluorescent staining provided by CellTraceā¢ Far Red dye enables the visualization of six or more generations of proliferating cells before the signal is overwhelmed by intrinsic cellular auto-fluorescence. This kit enables consistent, homogeneous staining results with very little fluorescence variation between cells in a population, so distinct generations can be seen without any requirement for complex modeling software.
Long-term signal retention
Unlike stains that label the lipid membrane of cells, CellTraceā¢ Far Red stain easily crosses the plasma membrane and covalently binds inside cells where the stable, well-retained fluorescent dye offers a consistent signal, even after several days in a cell culture environment. CellTraceā¢ Far Red dye binds covalently to all free amines on the surface and inside of cells and shows little cytotoxicity, with minimal observed effect on the proliferative ability or biology of cells.
Easy multiplexing with other fluorophores
The red excitation at 630 nm and emission at 661 nm of CellTraceā¢ Far Red dye make it ideal for multiplexing due to the limited spectral overlap with other common dyes (Alexa Fluorā¢ 488, FITC, and RPE) and fluorescent proteins (Green Fluorescent Protein (GFP) and mCherry).
Simple, robust staining protocol
The CellTraceā¢ Far Red Cell Proliferation Kit contains convenient single-use vials of dry dye to permit small-scale experiments without preparing excess quantities of dye. A stock solution is prepared by dissolving the contents of a vial in anhydrous DMSO prior to use. To stain 1 mL of cells in protein-free medium, 1Ī¼L of this stock solution is typically used. Cells should be stained for 20 minutes at room temperature with gentle agitation. A brief wash with complete medium will then quench any dye remaining in solution.
Specifications
| Continas 9 vials of CellTraceā¢ Far Red (lyophilized powder) and 1 vial of DMSO (500 Ī¼L). Store in freezer (-5 to -30Ā°C). | |
| Proliferation Assay | |
| Cell Proliferation Kit | |
| 633/635 | |
| Tube(s) | |
| 180 Assays |
| Fluorescence | |
| Flow Cytometer | |
| Contains 9 vials of CellTraceā¢ Far Red (lyophilized powder) and 1 vial of DMSO (500Ī¼L) | |
| Lyophilized | |
| CellTrace | |
| Room Temperature |
For Research Use Only. Not for use in diagnostic procedures.