Invitrogen™ eBioscience™ Cell Proliferation Dye eFluor™ 450

• A violet fluorescent dye that can be used to monitor individual cell divisions
• Dye binds to any cellular proteins containing primary amines, and as cells divide, the dye is distributed equally between daughter cells
• They can be measured as successive halving of the fluorescence intensity of the dye - visualize up to 7 generations
• Cells labeled with Cell Proliferation Dye eFluor™ 450 may be fixed and permeabilized for analysis of intracellular targets using standard formaldehyde-containing fixatives and saponin-based permeabilization buffers
• Cell Proliferation Dye eFluor™ 450 has a peak excitation of 409 nm and can be detected using a 450/50 band pass filter (equivalent to eFluor™ 450 or Pacific Blue™), making it compatible with applications that utilize GFP

Additional Details

• Each vial may be reconstituted to a stock concentration of 10 mM with 165 μL of anhydrous DMSO
• Once reconstituted it should be used within 6 months and protected from light and stored at -20°C with dessicant
• Avoid freeze-thawing