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Invitrogen™ eBioscience™ Cell Proliferation Dye eFluor™ 450
A violet fluorescent dye that can be used to monitor individual cell divisions for up to 7 generations.
Supplier: Invitrogen™ 65084285
Description
- A violet fluorescent dye that can be used to monitor individual cell divisions
- Dye binds to any cellular proteins containing primary amines, and as cells divide, the dye is distributed equally between daughter cells
- They can be measured as successive halving of the fluorescence intensity of the dye - visualize up to 7 generations
- Cells labeled with Cell Proliferation Dye eFluor™ 450 may be fixed and permeabilized for analysis of intracellular targets using standard formaldehyde-containing fixatives and saponin-based permeabilization buffers
- Cell Proliferation Dye eFluor™ 450 has a peak excitation of 409 nm and can be detected using a 450/50 band pass filter (equivalent to eFluor™ 450 or Pacific Blue™), making it compatible with applications that utilize GFP
Additional Details
- Each vial may be reconstituted to a stock concentration of 10 mM with 165 μL of anhydrous DMSO
- Once reconstituted it should be used within 6 months and protected from light and stored at -20°C with dessicant
- Avoid freeze-thawing
Spécifications
| Violet | |
| Proliferation Assay | |
| Dye | |
| Lyophilized powder | |
| 500 μg |
| Fluorescence | |
| Flow Cytometer | |
| 405/450 | |
| eBioscience |
For Research Use Only. Not for use in diagnostic procedures.