Invitrogen™ CD62L (L-Selectin) Monoclonal Antibody (MEL-14), Brilliant Ultra Violet™ 496, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

The MEL-14 mAb reacts with mouse CD62L, a 76 kDa member of the selectin family. CD62L is expressed by neutrophils, monocytes, and subsets of T, B, and NK cells and binds a number of glycosylated, fucosylated, sulfated sialylated glycoproteins including CD34, glycam-1 and MAdCam-1. These interactions mediate rolling of lymphocytes on activated endothelium at the sites of inflammation and homing of cells to the high endothelial venules (HEV) of peripheral lymphoid tissues. Applications Reported: This MEL-14 antibody has been reported for use in flow cytometric analysis. Applications Tested: This MEL-14 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.125μg per test Brilliant Ultra Violet 496 (BUV496) is a tandem dye that emits at 496 nm and is intended for use on cytometers equipped with an Ultraviolet (355 nm) laser. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright, Brilliant Violet, Brilliant Ultra Violet, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer (00-4409-75) to minimize any non-specific polymer interactions. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Excitation: 350 nm; Emission: 496 nm; Laser: Ultraviolet Laser.