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  6. Invitrogen™ CD45.2 Monoclonal Antibody (104), PerCP-Cyanine5.5, eBioscience™, Invitrogen™

Invitrogen™ CD45.2 Monoclonal Antibody (104), PerCP-Cyanine5.5, eBioscience™, Invitrogen™

Catalog No. 5015800
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100 μg
25 μg
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5015800 25 μg
5015801 100 μg
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Catalog No. 5015800 Supplier Invitrogen™ Supplier No. 45045480

Description

Mouse Monoclonal Antibody

Description: The 104 monoclonal antibody reacts with the mouse CD45 molecule, the leukocyte common antigen (LCA) in CD45.2-expressing mouse strains. The strains that express CD45.2 include the most commonly used mouse strains C57BL/6, BALB/c, C58, DBA/1, DBA/2, C3H/He, CBA, 129, A and AKR. CD45.2 is expressed by all leukocytes in these strains. Applications Reported: This 104 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 104 antibody has been tested by flow cytometric analysis of BALB/c splenocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered.

CD45.2 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45.2 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45.2 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45.2 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells.

Specifications

Antigen CD45.2
Applications Flow Cytometry
Classification Monoclonal
Clone 104
Concentration 0.2 mg/mL
Conjugate PerCP-Cyanine5.5
Formulation PBS with 0.09% sodium azide; pH 7.2
Gene Ptprc
Gene Accession No. P06800
Gene Alias B220; Cd45; CD45 antigen; CD45R; GP180; LCA; L-CA; leukocyte common antigen; loc; LY5; Ly-5; lymphocyte antigen 5; lymphocyte common antigen; Lyt-4; protein tyrosine phosphatase, receptor type, C; Ptprc; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein
Gene Symbols Ptprc
Host Species Mouse
Purification Method Affinity chromatography
Quantity 25 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 19264
Target Species Mouse
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Product Type Antibody
Form Liquid
Isotype IgG2a κ
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For Research Use Only.