Invitrogen™ CD38 Monoclonal Antibody (HIT2), APC-eFluor™ 780, eBioscience™, Invitrogen™

Description

Description: The HIT2 monoclonal antibody reacts with the human CD38 molecule, an approximately 45 kDa type II transmembrane protein. CD38 is expressed by thymocytes, peripheral B cells including plasma cells, activated T cells, and monocytes. CD38 is a counter-receptor for CD31 and has both cyclase and hydrolase enzymatic activity. Applications Reported: This HIT2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This HIT2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation.

CD38 is a multifunctional ectoenzyme and type II transmembrane glycoprotein involved in various cellular processes. It catalyzes the conversion of NAD into secondary messengers such as nicotinic acid adenine dinucleotide phosphate (NAADP) and cyclic ADP-ribose (cADPR). CD38 plays a crucial role in B cell development, with expression levels fluctuating from high in immature cells to low in intermediate ones, and back to high in mature B cells. It is also present in a variety of tissues and hematopoietic cells, including T cells, NK cells, and monocytes, and is used to phenotype leukemias and monitor HIV-1 progression. The CD34+CD38- population is considered to define the most pluripotent hematopoietic stem cells. In addition to its surface expression, CD38 has been identified in the nucleus, where it may regulate calcium levels. It functions as a multi-catalytic ectoenzyme, serving roles as an ADP-ribosyl cyclase, cyclic ADP-ribose hydrolase, and possibly NAD+ glycohydrolase, or as a cell surface receptor. CD38 is involved in the activation, proliferation, and differentiation of mature lymphocytes and mediates apoptosis of myeloid and lymphoid progenitor cells. It also participates in cell adhesion, signal transduction, and calcium signaling. Antibodies to CD38 are valuable for subtyping lymphomas and leukemias, detecting plasma cells (such as in myelomas), and marking activated B and T cells. CD38 is expressed at high levels in the pancreas, liver, kidney, malignant lymphoma, and neuroblastoma. Dysfunctions in CD38 are associated with diseases like chronic lymphocytic leukemia and Richter's Syndrome.