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  6. Invitrogen™ CD305 (LAIR1) Monoclonal Antibody (NKTA255), NovaFluor™ Yellow 660, eBioscience™

Invitrogen™ CD305 (LAIR1) Monoclonal Antibody (NKTA255), NovaFluor™ Yellow 660, eBioscience™

Catalog No. H081T03Y04A
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Description

Mouse Monoclonal Antibody

This NKTA255 monoclonal antibody reacts with human CD305, which is also known as leukocyte-associated Ig-like receptor (LAIR) 1. The NKTA255 antibody has been reported to inhibit NK cytolytic activity. This product contains 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer. Applications Tested This NKTA255 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood cells. This can be used at 5 μL (0.6 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Master mixes Master mixes of NFs should be made at 2-8 °C and may be made up to 4 hours ahead of time. We do not recommend storing master mixes containing NovaFluor conjugates overnight or longer. Whole Blood compatibility When utilizing whole blood (as opposed to density-gradient-purified PBMC), we recommend lysing red blood cells in bulk prior to staining with NovaFluor conjugates. See the Bulk Lysis of Human Whole Blood protocol here. Staining of whole blood with NovaFluor conjugates followed by lysis of red blood cells may result in higher-than-expected background staining. Viability dye compatibility NovaFluor dyes are not compatible with DNA intercalating viability dyes.....

CD305, also known as LAIR1, is a type I transmembrane glycoprotein with an extracellular Ig-like domain and two intracellular ITIMs. It is expressed on a wide range of immune cells, including T, NK, and B cells, monocytes, dendritic cells, eosinophils, basophils, and mast cells. In humans, CD305 is also present on CD34+ hematopoietic progenitor cells and thymocytes. Several isoforms exist due to alternative splicing, with expression varying between cell types. CD305 functions as an inhibitory receptor, playing a key role in regulating immune responses. It blocks cytokine-mediated signaling, cell proliferation, and apoptosis. In NK, B, and T cells, CD305 modulates cytolytic functions and cytokine production, down-regulating IL2 and IFNG in CD4+ T cells while inducing transforming growth factor beta. In B cells, it reduces IgG and IgE production and decreases IL8, IL10, and TNF secretion. Activation of CD305 through tyrosine phosphorylation recruits phosphatases PTPN6 and PTPN11, although it may also function independently of SH2-containing phosphatases. It inhibits differentiation of peripheral blood precursors into dendritic cells and has been shown to induce apoptosis in myeloid leukemia cell lines. Collagens are identified as ligands for CD305, underscoring its role in immune regulation.

Specifications

Antigen CD305 (LAIR1)
Applications Flow Cytometry
Classification Monoclonal
Clone NKTA255
Concentration 0.6 μg/Test
Conjugate NovaFluor Yellow 660
Formulation PBS with BSA and 0.09% sodium azide; pH 7.2
Gene LAIR1
Gene Accession No. Q6GTX8
Gene Alias 5133400O11Rik; BB115266; CD305; D7Bwg0421e; immunoglobulin heavy chain variable region; Lair1; Lair-1; leukocyte associated immunoglobulin like receptor 1; leukocyte-associated Ig-like receptor 1; leukocyte-associated Ig-like receptor-1; leukocyte-associated immunoglobulin-like receptor 1; rLAIR1
Gene Symbols LAIR1
Host Species Mouse
Purification Method Affinity chromatography
Quantity 100 Tests
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 3903
Target Species Human
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Product Type Antibody
Form Liquid
Isotype IgG1
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