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  6. Invitrogen™ CD289 (TLR9) Monoclonal Antibody (M9.D6), FITC, eBioscience™, Invitrogen™

Invitrogen™ CD289 (TLR9) Monoclonal Antibody (M9.D6), FITC, eBioscience™, Invitrogen™
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Catalog No. 501122170
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25 μg
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501122170 100 μg
5010061 25 μg
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Catalog No. 501122170 Supplier Invitrogen™ Supplier No. 11909382

Description

Rat Monoclonal Antibody

Description: M9.D6 is generated against a peptide derived from the extracellular portion of mouse TLR9. Predomitly expressed as an intracellular protein, TLR9 is a ∽115-120 kDa molecule which mediates response to unmethylated CpG dinucleotides in bacterial DNA. CpG DNA induces a strong T-helper-1-like inflammatory response and the proliferation of TLR9+ B cells. When stimulated with CpG DNA, TLR9-deficient (TLR9-/-) mice lacked splenocyte proliferation, inflammatory cytokine production from macrophages, and dendritic cell maturation, as compared with normal mice. To date, at least ten members of the Toll family have been identified. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Members of the TLR family are involved in recognition and response to different microbial components including lipoproteins, peptidoglycans, and nucleic acids and play important roles in innate immunity and inflammation. TLR9 is not detected by flow cytometry using this antibody on RBC-lysed mouse splenocytes stained for intracellular TLR9. This may be due to limitations of antigen detection by flow cytometry. Further studies are needed to determine the relationship between mRNA expression and protein detection. Applications Reported: This M9.D6 antibody has been reported for use in intracellular flow cytometric analysis. Applications Tested: This M9.

The protein encoded by this gene is a member of the Toll-like receptor family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is preferentially expressed in immune cell rich tissues, such as spleen, lymph node, bone marrow and peripheral blood leukocytes. Studies in mice and human indicate that this receptor mediates cellular response to unmethylated CpG dinucleotides in bacterial DNA to mount an innate immune response.

Specifications

Antigen CD289 (TLR9)
Applications Flow Cytometry
Classification Monoclonal
Clone M9.D6
Concentration 0.5 mg/mL
Conjugate FITC
Formulation PBS with 0.09% sodium azide; pH 7.2
Gene Tlr9
Gene Accession No. Q9EQU3
Gene Alias CD289; Tlr9; toll like receptor 9; toll-like receptor 9; UNQ5798/PRO19605
Gene Symbols Tlr9
Host Species Rat
Purification Method Affinity chromatography
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 81897
Target Species Mouse
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Product Type Antibody
Form Liquid
Isotype IgG2a κ
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For Research Use Only.