Invitrogen™ CD28 Monoclonal Antibody (37.51), eBioscience™, Invitrogen™

Syrian Hamster Monoclonal Antibody

Description: The 37.51 monoclonal antibody reacts with the mouse CD28 molecule, a 45 kDa homodimer expressed by thymocytes, mature T cells and NK cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) and is a potent costimulator of T cells. Signaling through CD28 augments IL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells. Applications Reported: The 37.51 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining. 37.51 has also been reported in costimulation of T cells in vitro and in vivo . (Please use Functional Grade purified 37.51, cat. 16-0281, in functional assays.). Applications Tested: The 37.51 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Note: It has been observed that increased incubations times (30-45 minutes) with the CD28 antibody can enhance the staining obtained. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.