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  6. Invitrogen™ CD1d Monoclonal Antibody (1B1), NovaFluor™ Yellow 610, eBioscience™

Invitrogen™ CD1d Monoclonal Antibody (1B1), NovaFluor™ Yellow 610, eBioscience™

Catalog No. M049T03Y03A
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M049T03Y03A 100 μg
M049T02Y03A 25 μg
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Catalog No. M049T03Y03A Supplier Invitrogen™ Supplier No. M049T03Y03A
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Description

Rat Monoclonal Antibody

Description The 1B1 monoclonal antibody reacts with mouse CD1d, a 48 kDa glycoprotein with structural homology to MHC class I molecules. While similar to MHC Class I, CD1d associates with beta2m, functionally CD1d is similar to MHC Class II. 1B1 detects CD1d at varying levels on mouse leukocytes. 1B1 detects beta2m associated CD1d. This product contains 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer. Applications Tested The 1B1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.8 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Master mixes Master mixes of NFs should be made at 2-8 °C and may be made up to 4 hours ahead of time. We do not recommend storing master mixes containing NovaFluor conjugates overnight or longer. Whole Blood compatibility When utilizing whole blood (as opposed to density-gradient-purified PBMC), we recommend lysing red blood cells in bulk prior to staining with NovaFluor conjugates. See the Bulk Lysis of Human Whole Blood protocol here. Staining of whole blood with NovaFluor conjugates follow...

CD1d is a heavy chain associated with Beta 2 microglobulin on cortical thymocytes. Beta 2 microglobulin independent expression of CD1d has also been demonstrated on human intestinal epithelial cells. CD1d is the sole group 2 member of the CD1 family of major histocompatibility (MHC) like glycoproteins. The CD1d gene encodes a divergent member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by the CD1d gene localizes to late endosomes and lysosomes via a tyrosine-based motif in the cytoplasmic tail. Diseases associated with CD1D include Mycobacterium Malmoense and Autoimmune Disease.

Specifications

Antigen CD1d
Applications Flow Cytometry
Classification Monoclonal
Clone 1B1
Concentration 0.1 mg/mL
Conjugate NovaFluor Yellow 610
Formulation PBS with BSA and 0.09% sodium azide; pH 7.2
Gene Cd1d1
Gene Accession No. P11609
Gene Alias AI747460; Antigen-presenting glycoprotein CD1d; antigen-presenting glycoprotein CD1d1; Cd1; CD1.1; CD1A; Cd1d; CD1D antigen; CD1D antigen, d polypeptide; CD1d molecule; CD1d.1; Cd1d1; CD1d1 antigen; CD1d1 molecule; differentiation antigen CD1-alpha-3; HMC class I antigen-like glycoprotein CD1D; Ly-38; R3; R3G1; T-cell surface glycoprotein CD1d; T-cell surface glycoprotein CD1d1; thymocyte antigen CD1D
Gene Symbols Cd1d1
Host Species Rat
Purification Method Affinity chromatography
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 12479
Target Species Mouse
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Product Type Antibody
Form Liquid
Isotype IgG2b κ
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