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  6. Invitrogen™ CD183 (CXCR3) Monoclonal Antibody (CEW33D), NovaFluor™ Red 685, eBioscience™

Invitrogen™ CD183 (CXCR3) Monoclonal Antibody (CEW33D), NovaFluor™ Red 685, eBioscience™

Catalog No. H087T02R02A
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Description

Mouse Monoclonal Antibody

The CEW33D monoclonal antibody reacts with human CD183. CD183, also known as CXCR3, is a G protein-coupled chemokine receptor that interacts with ligands CXCL9 (MIG), CXCL10 (IP-10), and CXCL11 (I-TAC). This product contains 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer. Applications Tested This CEW33D antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.6 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Master mixes Master mixes of NFs should be made at 2-8 °C and may be made up to 4 hours ahead of time. We do not recommend storing master mixes containing NovaFluor conjugates overnight or longer. Whole Blood compatibility When utilizing whole blood (as opposed to density-gradient-purified PBMC), we recommend lysing red blood cells in bulk prior to staining with NovaFluor conjugates. See the Bulk Lysis of Human Whole Blood protocol here. Staining of whole blood with NovaFluor conjugates followed by lysis of red blood cells may result in higher-than-expected background staining. Viability dye compatibility NovaFluor dyes are not compatible with DNA intercalating viability d...

CD183, also known as CXCR3, is a seven-transmembrane G protein-coupled chemokine receptor that binds CXCL9 (Mig), CXCL10 (IP-10), and CXCL11 (I-TAC), which are part of the CXC chemokine subfamily. CD183 plays a crucial role in leukocyte traffic, influencing integrin activation, cytoskeletal changes, and chemotactic migration. It is expressed on NK cells, subsets of T lymphocytes, regulatory T cells (Tregs), and is preferentially found on Th1-polarized cells. CD183 is prominently expressed in effector/memory T cells and T cells in inflamed tissues, contributing to CD4 T cell responses to grafts, as evidenced by compromised allograft rejection in CXCR3 knockout mice. Chemokine binding induces rapid, short-lived cellular responses due to receptor internalization, with responsiveness restored after receptor recycling. Inhibition by Bordetella pertussis toxin suggests coupling with Gi subclass G proteins. The production of IP-10, Mig, and I-TAC in inflammatory lesions indicates CD183 role in recruiting inflammatory cells, making it a target for developing antagonists to treat inflammatory diseases. Multiple transcript variants encoding different isoforms of CD183 have been identified.

Specifications

Antigen CD183 (CXCR3)
Applications Flow Cytometry
Classification Monoclonal
Clone CEW33D
Concentration 0.6 μg/Test
Conjugate NovaFluor Red 685
Formulation PBS with BSA and 0.09% sodium azide; pH 7.2
Gene CXCR3
Gene Accession No. P49682
Gene Alias an; C Cmotif chemokine; C X C motif chemokine; CC motif chemokine; CCmotif chemokine; CD182; CD183; chemokine (C-X-C motif) receptor 3; chemokine receptor 3; CKR-L2; Cmkar3; CXC; C-X-C chemokine receptor type 3; CXC motif chemokine; C-X-C motif chemokine receptor 3; Cxcr3; CXC-R3; CXCR-3; G protein-coupled receptor 9; GPR9; Interferon-inducible protein 10 receptor; IP10; IP10 receptor; IP-10 receptor; IP10-R; Mig receptor; MigR; Mig-R
Gene Symbols CXCR3
Host Species Mouse
Purification Method Affinity chromatography
Quantity 25 Tests
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 2833
Target Species Human
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Product Type Antibody
Form Liquid
Isotype IgG1 κ
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