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CD123 Monoclonal Antibody (6H6), Brilliant Violet™ 480, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Specifications

Antigen CD123
Clone 6H6
Concentration 5 μL/Test
Applications Flow Cytometry
Classification Monoclonal
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Products 2
Catalog Number Mfr. No. Quantity Price Quantity  
Catalog Number Mfr. No. Quantity Price Quantity  
41-412-3942
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Invitrogen™
414123942
100 Tests N/A
N/A
Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.
41-412-3941
View Documents
Invitrogen™
414123941
25 Tests N/A
N/A
Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.
Description

Description

CD123 Monoclonal antibody specifically detects CD123 in Human samples. It is validated for Flow Cytometry

Description: The 6H6 monoclonal antibody reacts with human CD123, the alpha chain of the IL-3 receptor. This 60-70 kDa transmembrane protein binds to IL-3 with low affinity by itself, and when associated with CD131 (common beta chain) binds IL-3 with high affinity. CD123 is expressed by myeloid precursors, macrophages, dendritic cells, mast cells, basophils, and megakaryocytes. Applications Reported: This 6H6 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 6H6 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Brilliant Violet™ 480 (BV480) is a dye that emits at 479 nm and is intended for use on cytometers equipped with a violet (405 nm) laser. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information. Our internal testing suggests that Brilliant Violet™ 480 (BV480) is not compatible with methanol-based fixation. Excitation: 440 nm; Emission: 479 nm; Laser: Violet Laser. BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen.™
Specifications

Specifications

CD123
5 μL/Test
Monoclonal
Liquid
RUO
PBS with BSA and 0.09% sodium azide; pH 7.2
CD123; CD123 antigen; CDw123; Cyrl; cytokine receptor-like protein CYRL; hIL-3Ra; Il-3 alpha subunit; IL-3 receptor alpha chain; IL-3 receptor alpha SP2 isoform; IL-3 receptor subunit alpha; IL3R; IL-3R subunit alpha; Il3ra; IL-3RA; IL-3R-alpha; IL3RAY; IL3RX; IL3RY; interleukin 3 receptor subunit alpha; interleukin 3 receptor, alpha; interleukin 3 receptor, alpha (low affinity); interleukin 3 receptor, alpha chain; interleukin-3 receptor class II alpha chain; Interleukin-3 receptor subunit alpha; Sut-1
IgG1 κ
Affinity chromatography
Antibody
6H6
Flow Cytometry
Brilliant Violet 480
Mouse
Human
P26951
3563
Primary
4°C, store in dark, DO NOT FREEZE!
IL3RA
Videos
SDS
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