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Invitrogen™ Carboxypeptidase Y Monoclonal Antibody (10A5B5)
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Mouse Monoclonal Antibody

Supplier:  Invitrogen™ A6428

Encompass

Catalog No. A6428


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Description

Description

Antibody binding specificity was determined by Particle Concentration Fluorescence Immunoassay (PCFIA) using the yeast caboxypeptidase, by Western blot immunoassay using protein extracts from yeast, and by indirect immunofluorescence of fixed yeast cells.The antibody can be used to detect the yeast carboxypeptidase on Western blots or to immunolocalize the carboxypeptidase in fixed yeast cells. The antibody is ideal for screening for caroboxypeptidase secretion (Vps-phenotype) in yeast using the colony immunoblot overlay assay. The antibody has been extensively used in screens for new vps mutations, as well as in complementation analysis with the vps mutant collection.

Carboxypeptidase Y is Involved in the degradation of small peptides. It digests peptides containing an aliphatic or hydrophobic residue in P1' position, as well as methionine, leucine or phenylalanine in the P1 position of an ester substrate.
Specifications

Chemical Identifiers

Carboxypeptidase Y
1 mg/mL
Western Blot, Immunocytochemistry
Unconjugated
Mouse
RUO
HEPES buffered saline with 0.02% sodium azide
P00729
855343
Purified yeast CPY (carboxypeptidase).
Antibody
10A5B5
4°C, do not freeze
Monoclonal
Liquid
IgG2a κ
Yeast
PRC1
carboxypeptidase C PRC1; Carboxypeptidase Y; Carboxypeptidase YSCY; CPD-Y; CPY1; LBC1; pcy1; PRC1; YMR297W
PRC1
Primary
Ig fraction

Specifications

Carboxypeptidase Y
Monoclonal
1 mg/mL
HEPES buffered saline with 0.02% sodium azide
P00729
PRC1
Purified yeast CPY (carboxypeptidase).
250 μg
Primary
Yeast
Antibody
IgG2a κ
Western Blot, Immunocytochemistry
10A5B5
Unconjugated
PRC1
carboxypeptidase C PRC1; Carboxypeptidase Y; Carboxypeptidase YSCY; CPD-Y; CPY1; LBC1; pcy1; PRC1; YMR297W
Mouse
Ig fraction
RUO
855343
4°C, do not freeze
Liquid
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