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Invitrogen™ eBioscience™ Calcium Sensor Dye eFluor™ 514
Membrane-permeable dye used to monitor changes in intracellular free calcium concentrations in the cell. Once eFluor™ 514 Calcium Sensor Dye enters the cell, cellular esterases cleave the AM group yielding a membrane-impermeable dye fluorescing at ≈520nm.
Supplier: Invitrogen™ 65085939
Description
- Calcium binding affinity: Kd = 232 nM
- Molecular Weight: ≈1100 d
- Peak Excitation: 490nm
- Peak Emission: 514nm
- Reconstitute in high-quality, freshly opened DMSO
- Recommended concentration: 2 to 5 mM
- Once reconstituted, protect from light and store dessicated at −20°C; avoid freeze-thawing
Specifications
| Fluorescence | |
| Fluorescence Microscope, Flow Cytometer | |
| 490/514 | |
| 1mg |
| Calcium Assay | |
| Dye | |
| eBioscience |
For Research Use Only. Not for use in diagnostic procedures.