missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Cell Analysis Products
  4. Cell Based Assays
  5. Cell Function Reagents and Kits
  6. 5016954

Invitrogen™ eBioscience™ Calcein Violet 450 AM Viability Dye

Catalog No. 5016954
Change view
Click to view available options
Quantity:
1 mg
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
5016954 1 mg
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. 5016954 Supplier Invitrogen™ Supplier No. 65085439
Only null left

Description

Membrane-permeable live-cell labeling dye. Upon entering the cell, intracellular esterases cleave the acetoxymethyl (AM) ester group, yielding the membrane-impermeable Calcein Violet fluorescent dye. Apoptotic and dead cells with compromised cell membranes do not retain Calcein Violet 450.

  • Molecular weight: 600g/mol
  • Peak excitation: 408nm
  • Peak emission: 450nm
  • Reconstitute in high-quality, freshly opened DMSO
  • Once reconstituted, store at −20°C with dessicant and use within a short period of time. Avoid freeze-thawing
  • For fluorescent microscopy, detect using the appropriate filter sets
  • For flow cytometric analysis, excite with the violet laser line (405nm) and detect using filters for Pacific Blue™/eFluor™ 450 (450/50)
  • Co-staining with Annexin V or 7-AAD is recommended to allow the greatest resolution between live and dead/apoptotic cells

Specifications

Color Violet
Detection Method Fluorescence
For Use With (Application) Viability Assay
For Use With (Equipment) Fluorescence Microscope, Flow Cytometer
Product Type Dye
Emission 408/450
Product Line eBioscience
Quantity 1 mg

For Research Use Only. Not for use in diagnostic procedures.