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  6. Invitrogen™ eBioscience™ Calcein AM Viability Dye (UltraPure Grade)

Invitrogen™ eBioscience™ Calcein AM Viability Dye (UltraPure Grade)

Catalog No. 5016953
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20 x 50 μg
1 mg
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5016953 20 x 50 μg
5016952 1 mg
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Catalog No. 5016953 Supplier Invitrogen™ Supplier No. 65085378
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Description

A membrane-permeable live-cell labeling dye. Upon entering the cell, intracellular esterases cleave the acetoxymethyl (AM) ester group, yielding the membrane-impermeable Calcein fluorescent dye.

  • Upon entering the cell, intracellular esterases cleave the acetoxymethyl (AM) ester group, yielding the membrane-impermeable Calcein fluorescent dye
  • Apoptotic and dead cells with compromised cell membranes do not retain Calcein
  • Calcein is optimally excited at 495 nm and has a peak emission of 515 nm
  • For flow cytometric analysis, it can be excited off the blue laser line (488 nm) and detected using filters for FITC/Alexa Fluor™ 488 (530/30)
  • For multicolor flow cytometry, concentrations in the range of 5 to 20 nM are recommended to ensure that compensation can be performed successfully
  • Co-staining with Annexin V or 7-AAD is recommended to allow the greatest resolution between live and dead/apoptotic cells
  • If single-color analysis is desired, improved resolution between live and dead/apoptotic cells may be obtained by using either Calcein Blue AM (cat. 65-0855) or Calcein Violet 450 AM (cat. 65-0854)
  • Calcein AM should be reconstituted in high-quality, freshly opened DMSO
  • Once reconstituted, it should be stored at -20°C with dessicant and used within a short period of time (avoid freeze-thawing)
  • Purity: >95% (determined by HPLC)

Specifications

Color Green
Detection Method Fluorescence
For Use With (Application) Viability Assay
For Use With (Equipment) Fluorescence Microscope, Flow Cytometer
Product Type Dye
Emission 495/515
Product Line eBioscience
Quantity 20 x 50 μg

For Research Use Only. Not for use in diagnostic procedures.