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Cytiva Butyl-S Sepharose™ 6 Fast Flow Chromatography Media

Catalog No. 45002941
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200 mL
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Catalog No. Quantity
45002941 200 mL
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Catalog No. 45002941 Supplier Cytiva Supplier No. 17097802
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Description

The least hydrophobic medium in the Sepharose Fast Flow hydrophobic interaction chromatography (HIC) range and is particularly useful during the initial stages of purification process

  • Binds and elutes relatively strong hydrophobic molecules at comparatively low salt concentrations
  • Hydrophilic nature of the base matrix ensures low levels of non-specific binding
  • Purifies recombinant Hepatitis B virus surface antigen from CHO cells
  • Composed of cross-linked 6% agarose beads modified with butyl groups via thio-ether linkage
  • Available in range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development

Specifications

Quantity 200 mL
For Use With (Application) For Hydrophobic Medium in The Sepharose Fast Flow Hydrophobic Interaction Chromatography (HIC) Range and is Particularly Useful During The Initial Stages of PurifiCatIon Process
Stability Stable in commonly used aqueous Buffers: 1 mM HCl, 1 M NaOH, 30% Isopropanol, 50% Ethylene Glycol, 70% Ethanol, 6 M Guanidine hydrochloride, 8 M Urea
Storage Requirements 4°C to 30°C
Product Type Butyl-S Sepharose 6 Fast Flow Media
Flow Rate 250 to 400 cm/Hoμr
Particle Size 45 to 165 μm
Max. Pressure 100 kPa
Matrix 6% Cross-Linked Agarose
pH Range 3 to 13
Storage Buffer 20% Ethanol
Ligand Type Butyl-S
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