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Thermo Scientific™ Bsm DNA Polymerase, large fragment (8 U/μL)

Thermo Scientific Bsm DNA Polymerase, Large Fragment, is equivalent to Bst DNA polymerase, which catalyzes 5' → 3' synthesis of DNA and lacks 5' → 3' and 3' → 5' exonuclease activities.

Supplier:  Thermo Scientific™ EP0691

Catalog No. FEREP0691



Description

Description

Thermo Scientific Bsm DNA Polymerase, Large Fragment, is equivalent to Bst DNA polymerase, which catalyzes 5' → 3' synthesis of DNA and lacks 5' → 3' and 3' → 5' exonuclease activities. Sourced from E. coli cells with a cloned polA gene from Bacillus smithii, Bsm DNA Polymerase, Large Fragment, features strong strand displacement activity and is active in a wide range of temperatures (30–63°C), with an optimum of activity at 60°C. With high functional similarity to Bst DNA Polymerase, Large Fragment, this enzyme can serve as alternative in most applications including loop-mediated isothermal amplification (LAMP).

Thermo Scientific Bsm DNA Polymerase, Large Fragment, is equivalent to Bst DNA polymerase, which catalyzes 5' → 3' synthesis of DNA and lacks 5' → 3' and 3' → 5' exonuclease activities. Sourced from E. coli cells with a cloned polA gene from Bacillus smithii, Bsm DNA Polymerase, Large Fragment, features strong strand displacement activity and is active in a wide range of temperatures (30–63°C), with an optimum of activity at 60°C. With high functional similarity to Bst DNA Polymerase, Large Fragment, this enzyme can serve as an alternative in most applications, including loop-mediated isothermal amplification (LAMP).

Applications
Bsm DNA Polymerase, Large Fragment, may be used in a wide variety of applications including:
• Loop-mediated isothermal amplification (LAMP)
• Whole genome amplification (WGA)
• Ramification amplification (RAM)
• Random-primed DNA labeling
• Labeling by fill-in 5'-overhangs of dsDNA

Specifications

Specifications

Stand-alone enzyme
Bsm DNA Polymerase

• Bsm DNA Polymerase, Large Fragment (200 μL at 8 U/μL)
• 10X Bsm Buffer (1.25 mL)

Store at –15 to –25°C.

Dry Ice
30 to 60 minutes
LAMP, RT-LAMP, WGA, RAM
1600 U
60°C
8 U/μL
SDS
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For Research Use Only. Not for use in diagnostic procedures.