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Thermo Scientific™ Bsm DNA Polymerase, large fragment (8 U/μL)

Catalog No. FEREP0691
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Quantity:
1,600 units
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Catalog No. Quantity
FEREP0691 1,600 units
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Catalog No. FEREP0691 Supplier Thermo Scientific™ Supplier No. EP0691
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Description

Thermo Scientific Bsm DNA Polymerase, Large Fragment, is equivalent to Bst DNA polymerase, which catalyzes 5' → 3' synthesis of DNA and lacks 5' → 3' and 3' → 5' exonuclease activities.

Thermo Scientific Bsm DNA Polymerase, Large Fragment, is equivalent to Bst DNA polymerase, which catalyzes 5' → 3' synthesis of DNA and lacks 5' → 3' and 3' → 5' exonuclease activities. Sourced from E. coli cells with a cloned polA gene from Bacillus smithii, Bsm DNA Polymerase, Large Fragment, features strong strand displacement activity and is active in a wide range of temperatures (30–63°C), with an optimum of activity at 60°C. With high functional similarity to Bst DNA Polymerase, Large Fragment, this enzyme can serve as alternative in most applications including loop-mediated isothermal amplification (LAMP).

Thermo Scientific Bsm DNA Polymerase, Large Fragment, is equivalent to Bst DNA polymerase, which catalyzes 5' → 3' synthesis of DNA and lacks 5' → 3' and 3' → 5' exonuclease activities. Sourced from E. coli cells with a cloned polA gene from Bacillus smithii, Bsm DNA Polymerase, Large Fragment, features strong strand displacement activity and is active in a wide range of temperatures (30–63°C), with an optimum of activity at 60°C. With high functional similarity to Bst DNA Polymerase, Large Fragment, this enzyme can serve as an alternative in most applications, including loop-mediated isothermal amplification (LAMP).

Applications
Bsm DNA Polymerase, Large Fragment, may be used in a wide variety of applications including:
• Loop-mediated isothermal amplification (LAMP)
• Whole genome amplification (WGA)
• Ramification amplification (RAM)
• Random-primed DNA labeling
• Labeling by fill-in 5'-overhangs of dsDNA

Specifications

Product Type Stand-alone enzyme
For Use With (Application) LAMP, RT-LAMP, WGA, RAM
Polymerase Bsm DNA Polymerase
Quantity 1,600 units
Content And Storage

• Bsm DNA Polymerase, Large Fragment (200 μL at 8 U/μL)
• 10X Bsm Buffer (1.25 mL)

Store at –15 to –25°C.
Optimal Reaction Temperature 60°C
Shipping Condition Dry Ice
Concentration 8 U/μL
Reaction Time 30 to 60 minutes

For Research Use Only. Not for use in diagnostic procedures.