Invitrogen™ BrdU Monoclonal Antibody (IIB5)

Description

OMA1-06200 detects bromodeoxyuridine (BrdU). OMA1-06200 has been successfully used in immunocytochemistry, immunohistochemical and FACS procedures. This antibody specifically detects free BrdU and BrdU that has been incorporated into nuclear DNA. OMA1-06200 antigen is purified BrdU. Store at 4ºC, or in small aliquots at -20ºC.

Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues, and can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.