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Invitrogen™ eBioscience™ BrdU Kit for IHC/ICC Immunofluorescence eFluor™ 570

Catalog No. 501125104
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50 tests
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501125104 50 tests
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Contains Sodium azide and formaldehyde
Catalog No. 501125104 Supplier Invitrogen™ Supplier No. 8841659945
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Contains Sodium azide and formaldehyde

Description

Includes

  • BrdU Fixation Buffer
  • BrdU Antigen Retrieval Solution (10X)
  • BrdU Blocking Solution
  • Anti-BrdU Biotin (clone BU20A) : 10 μg
  • Streptavidin for BrdU IHC/ICC eFluor 570 : 2 μg

Contains reagents and buffers to identify and examine proliferating cells by immunohistochemical or immunocytochemical analysis. Optimized for IHC with both frozen and formalin-fixed paraffin-embedded BrdU-labeled mouse intestine and ICC of BrdU-pulsed HeLa cells grown on culture slides.

Cells are labeled in vitro or in vivo with 5-bromo-2’-deoxyuridine (BrdU), a synthetic analog of thymidine, which is incorporated into DNA in place of thymidine during the S-phase of the cell cycle. Following fixation and antigen retrieval steps, cells or tissue sections are stained for BrdU incorporation and visualized using a streptavidin-conjugated fluorophore.

Specifications

Conjugate BrdU
Detection Method Fluorometric
Quantity 50 tests
Product Line eBioscience
For Use With (Application) ICC (Immunochemistry), IHC (Immunohistochemistry)
Product Type IHC/ICC BrdU Kit
Target Species All species

For Research Use Only. Not for use in diagnostic procedures.