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  5. Invitrogen™ Bovine Insulin ELISA Kit

Invitrogen™ Bovine Insulin ELISA Kit

Catalog No. EB13RBX5
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5 x 96 Tests
96 Tests
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EB13RBX5 5 x 96 Tests
EB13RB 96 Tests
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Catalog No. EB13RBX5 Supplier Invitrogen™ Supplier No. EB13RBX5

Description

ELISA

Bovine Insulin quantitates bovine Insulin in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant bovine Insulin.

Insulin is a peptide hormone secreted by beta cells of the pancreatic islets. It regulates carbohydrate, protein and lipid metabolism by enhancing membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Insulin is 51-amino acid polypeptide product produced from a precursor peptide, proinsulin. Proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds and one molecule of C-peptide. Insulin deficiency results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of b-cell origin such as insulinoma.

Specifications

Accession Number P01308, P01315, P01317, P01322, P01323, P01325, P01326
Assay Range 4.5-300 μIU/mL
Assay Sensitivity 4.5 μIU/mL
Conjugate HRP
Immunoassay Kit Format Sandwich ELISA Kit
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
Target Species Human, Mouse, Rat, Bovine, Pig
For Use With (Equipment) Absorbance Microplate Reader
Gene Alias AA986540; IDDM; IDDM1; IDDM2; ILPR; INS; Ins1; Ins-1; Ins2; Ins-2; Ins2-rs1; InsII; Insulin; insulin 1; insulin 2; Insulin A chain; Insulin B chain; insulin I; insulin II; insulin-1; Insulin-1 A chain; Insulin-1 B chain; Insulin-2; Insulin-2 A chain; Insulin-2 B chain; IRDN; Mody; MODY10; Mody4; preproinsulin; proinsulinAA986540; IDDM; IDDM1; IDDM2; ILPR; INS; Ins1; Ins-1; Ins2; Ins-2; Ins2-rs1; InsII; Insulin; insulin 1; insulin 2; Insulin A chain; Insulin B chain; insulin I; insulin II; insulin-1; Insulin-1 A chain; Insulin-1 B chain; Insulin-2; Insulin-2 A chain; Insulin-2 B chain; IRDN; Mody; MODY10; Mody4; preproinsulin; proinsulin;
Gene ID (Entrez) 16333, 16334, 24505, 24506, 280829, 3630, 397415
Gene Symbol INS, Ins1, Ins2
Interassay CV <12%
Intraassay CV <10%
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye Biotin
Quantity 5 x 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Shipping Condition Wet or Dry Ice
Storage Requirements Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Target Insulin
Test Time 1 hr 20 min
Total Assay Time 4 hr 45 min
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