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Invitrogen™ BLOCK-iT™ Transfection Optimization Kit DFS Item

BLOCK-iT™ Transfection Optimization Kit

Supplier:  Invitrogen™ 13750047

Catalog No. 13750047


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Description

Description

The BLOCK-iT™ Transfection Optimization Kit is designed to optimize RNAi transfection using controls for transfection and viability. Each kit contains a BLOCK-iT™ Fluorescent Oligo, which provides:

  • A strong, easily detectable FITC signal indicating transfection efficiency
  • Chemical modifications for a clearer, more persistent cellular signal than labeled siRNA provides
  • Proven correlation of transfection efficiency with Stealth RNAi™ siRNA and traditional siRNA molecules

    This makes BLOCK-iT™ Fluorescent Oligo an ideal indicator of transfection efficiency for RNAi experiments. In addition, the kit contains a Positive Control Stealth RNAi™ siRNA molecule directed at human p53 and a Scrambled Stealth RNAi™ siRNA molecule as a negative control. To assay cell viability, Dead Cell Dye is included. This dye colors dead cells as red, thereby allowing easy identification of non-viable cells in a tissue culture plate. Using these reagents together allows you to both optimize transfection conditions and have a functional positive control in every RNAi experiment you perform.
    • Specifications

    Specifications

    Cell Viability Control, Negative Control, Transfection Control, Positive Control
    RNA
    Transfection
    Transfection Kit
    siRNA
    Kit
    pLATE
    BLOCK-iT™, Stealth RNAi™ siRNA
    1 Kit
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    For Research Use Only. Not for use in diagnostic procedures.