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Invitrogen™ BioNick™ DNA Labeling System

Catalog No. 18247015
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Catalog No. 18247015 Supplier Invitrogen™ Supplier No. 18247015
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Description

BioNick™ DNA Labeling System

The BioNick™ DNA Labeling System is ideal for generating biotinylated DNA probes by nick translation optimized for use in non-radioactive in situ hybridizations. These probes can also be used in Southern or northern blots, plaque lifts, colony hybridizations, and dot blot hybridizations. Using the BioNick™ DNA Labeling System:

  • DNA is labeled with biotin-14-dATP, producing probe sizes from 50 to 500 bp
  • One reaction labels 1μg of template DNA

    • Specifications

    Content And Storage The BioNick™ DNA Labeling System includes 10X dNTP mix containing biotin-14-dATP, 10X enzyme mix containing DNA Polymerase I and DNase I, control DNA, stop buffer, and distilled water. Store at -20°C. Guaranteed stable for 6 months when properly stored.
    Detection Method Biotin-based
    Label or Dye Biotin
    Final Product Type Probes (Labeled DNA)
    Labeling Target DNA (General)
    Includes Label or Dye Yes
    Labeling Method Direct Labeling
    Product Line BioNick
    Product Type DNA Labeling System
    Quantity 50 Reactions
    Shipping Condition Approved for shipment on Wet or Dry Ice
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    Frequently Asked Questions (FAQs)

    What is the difference between the BioNick system and the BioPrime system?

    The BioNick Labeling System generates biotinylated DNA probes by nick translation. These probes range from 50 to 500 bases. One reaction labels 1 µg of DNA. The BioPrime Labeling System generates 50-500 ng of biotinylated DNA from 25 ng template DNA by random priming. The probe size ranges from 50 to 700 bases, with a significant portion of the probe being less than 200 bases. See Mackey, J., Rashtchian, A. (1992) FOCUS 14, p.21.

    For Research Use Only. Not for use in diagnostic procedures.