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Invitrogen™ BacMam Enhancer Solution (1000X)

Catalog No. PV5835
Encompass
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150 μL
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PV5835 150 μL
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Catalog No. PV5835 Supplier Invitrogen™ Supplier No. PV5835

Description

BacMam Enhancer Solution

The BacMam Enhancer helps to increase expression of BacMam reagents in mammalian cells.

To use, simply add the BacMam enhancer to cell culture media with or without serum that have already been treated with the BacMam reagent, then incubate cells and replace with fresh medium. Different cell types vary in their response to the BacMam enhancer and for best results the amount and treatment time will need to be optimized.

Specifications

Content And Storage Concentration: 1.5 mM (1000X)
Store at -20°C.
Description Histone Deacetylase Inhibitor
Cell Type Mammalian Cells
Concentration 1000 X
Form Frozen
Product Type BacMam Enhancer Solution
For Use With (Application) BacMam-Enabled Cellular Assays
Quantity 150 μL
Shipping Condition Dry Ice

Frequently Asked Questions (FAQs)

I am using your CellLight BacMam reagents for fluorescent protein-labeling of my cell organelles, but they aren't labeling well. How can I improve it?

First, make sure that your cells are mammalian and non-blood derived. If the cells have low transduction efficiency (i.e., only a few cells are labeling or are labeling only dimly), first try increasing the particle number per cell. The next thing to try is to use the BacMam Enhancer Solution. If, however, your cells show labeling, but the localization seems to be too generalized or ubiquitous within the cell, then you should reduce the particle number per cell. The manual gives a guideline and formula for calculating particle number per cell.

For Research Use Only. Not for use in diagnostic procedures.