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Gibco™ B-27™ Supplement, minus insulin
Description
B-27 Supplement is suitable for use in most neuronal cell applications. For other applications, we provide alternative formulations of B-27 Supplement and Neurobasal media.
For established protocols using B-27 Supplement, access our Gibco Neurobiology Protocol Handbook.
View publications using Gibco neurobiology products at our Gibco CellCite.
Specifications
Specifications
| Content And Storage | Store in freezer (-5 to -20°C). Protect from light. |
| Cell Type | Neural Cells |
| Classification | Serum-free |
| Concentration | 50 X |
| Culture Type | Mammalian Cell Culture |
| Form | Liquid |
| Product Type | Cell Culture Supplement |
| Serum Level | Serum-Free |
| Sterility | Sterile-filtered |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
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Frequently Asked Questions (FAQs)
Yes, we do offer B-27 Supplement, minus insulin (Cat. Nos. A1895601 and A1895602). All our other B-27 supplements do contain insulin.
We do not recommend thawing at 37 degrees C. Best results are observed upon thawing at room temperature for approximately 2 hrs or overnight at 2-8 degrees C.
We recommend thawing it at 4 degrees C overnight or at room temperature for 2 hours. We don't recommend thawing in a water bath or incubator.
Yes, we recommend that you aliquot when you first thaw it and thereafter subject the aliquot to no more than 2 additional freeze/thaw cycles.
For Research Use Only. Not for use in diagnostic procedures.