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Gibco™ B-27™ Supplement (50X), minus antioxidants

Catalog No. 10889038
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10889038 10 mL
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Catalog No. 10889038 Supplier Gibco™ Supplier No. 10889038
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Description

B-27™ Supplement (50X), minus antioxidants

A variation of the most cited neuronal cell culture supplement, B-27 Supplement Minus Antioxidants (AO) is a customized version of the original B-27 Supplement without five antioxidants (vitamin E, vitamin E acetate, superoxide dismutase, catalase, and glutathione). B-27 Supplement Minus AO is a serum-free supplement designed for studies of the role of free-radicals in aging, toxicity, apoptosis, and chronic neurologic diseases. B-27 Supplement Minus AO is provided as a 50X liquid and is intended for use with Neurobasal Medium or Neurobasal-A Medium for neuronal cell culture without the need for an astrocyte feeder layer.

B-27 Supplement is suitable for use in most neuronal cell applications. For other applications, we provide alternative formulations of B-27 Supplement and Neurobasal media

Specifications

Content And Storage Store in freezer (-5 to -20°C) and protect from light.
Cell Type Neural Cells
Classification Serum-free
Concentration 50 X
Culture Type Mammalian Cell Culture
Form Liquid
Product Type Cell Culture Supplement
Serum Level Serum-Free
Sterility Sterile
pH 6 to 8
Product Line B-27
Quantity 10 mL
Shipping Condition Dry Ice
Source Serum-Free
Tested For Endotoxin, Performance, Sterility, Mycoplasma
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Frequently Asked Questions (FAQs)

Can B-27 supplement be filtered?

Yes, the protein content in this product is high enough so filtering through a low protein binding filter as a 50X or 1X in solution should not be a problem.

Can Neurobasal Medium alone or Neurobasal Medium supplemented with B-27 supplement be frozen to extend product shelf life?

No, it is not recommended to freeze media due to the potential of precipitates forming upon thaw. Inorganic salts and amino acids in the formulation may come out of solution when exposed to temperature fluctuations. These precipitates will not go back into solution easily once formed.
Do you offer B-27 supplements with and without insulin?

Yes, we do offer B-27 Supplement, minus insulin (Cat. Nos. A1895601 and A1895602). All our other B-27 supplements do contain insulin.
Can I thaw B-27 Supplements in a 37 degrees C water bath or incubator?

We do not recommend thawing at 37 degrees C. Best results are observed upon thawing at room temperature for approximately 2 hrs or overnight at 2-8 degrees C.
How do I thaw B-27 Supplements?

We recommend thawing it at 4 degrees C overnight or at room temperature for 2 hours. We don't recommend thawing in a water bath or incubator.
Can I re-freeze B-27 Supplements into smaller aliquots for future use?

Yes, we recommend that you aliquot when you first thaw it and thereafter subject the aliquot to no more than 2 additional freeze/thaw cycles.
What B-27 supplements do you offer?

We offer the following B-27 supplement products, which are developed for specific applications:

- Gibco B-27 Serum-free Supplement (Cat. No. 17504044): Complete formulation for the growth of long-term viability of neurons.
- Gibco B-27 Supplement minus AO (antioxidants) (Cat. No. 10889038): Five antioxidants are removed. The supplement is ideal in studies of oxidative damage, apoptosis, or applications in which free radical damage to neurons occurs.
- Gibco B-27 Supplement minus Insulin (Cat. No. A1895601): Insulin is removed. The supplement is ideal for studies of insulin secretion and insulin receptors.
- Gibco B-27 Supplement minus Vitamin A (Cat. No. 12587010): Vitamin A which induces neural differentiation is removed. The supplement is ideal for proliferation of stem cells.

For Research Use Only. Not for use in diagnostic procedures.