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Applied Biosystems™ AutoLys M Tubes and Caps

Catalog No. A38738
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A38738 25 each
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Catalog No. A38738 Supplier Applied Biosystems™ Supplier No. A38738
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Description

AutoLys M Tubes are for the preparation of FFPE samples for nucleic acid extraction.

A novel tube-in-tube design creates cleared lysates from FFPE tissue samples, without the need for deparaffinization or wash steps, while increasing DNA and RNA yields. Just add your FFPE sample to the tube, followed by protease buffer and enzyme from the MagMAX FFPE DNA/RNA Ultra Kit. Once digestion is complete, lift the tubes from the AutoLys M Tube Rack using the AutoLys M TubeLifter or AutoLys M Tube Pliers and spin down the samples using a benchtop centrifuge with plate holder (SBS standard format). The FFPE-associated wax and debris are held in the upper chamber of the tube while the lysate passes through. Nucleic acids can then be purified from the clarified lysate using the MagMAX FFPE DNA/RNA Ultra Kit.

Features of the AutoLys M Tube system include:
• FFPE sample lysis and nucleic acid separation from debris in a single tube
• 2-D barcode on bottom tube
• Elimination of use of organic solvents (xylene, CitriSolv) for deparaffinization and washes
• Reduced processing time
• Increased nucleic acid yield

The AutoLys M Tube system is a unique way to process your FFPE samples that will result in improved nucleic acid yields when used in conjunction with the MagMAX FFPE DNA/RNA Ultra Kit, helping ensure the success of downstream assays and reducing the time to data analysis.

Specifications

Sample Type FFPE Tissue
Content And Storage • 25 AutoLys M tubes
• 25 AutoLys M caps
For Use With (Equipment) AutoLys TubeLifter System
Quantity 25 each
Shipping Condition Room Temperature

Frequently Asked Questions (FAQs)

Can I use a heat block for the AutoLys M tube incubation?

No. The AutoLys M tubes don't fit in a standard 1.5 mL or 2.0 mL heat block. We recommend incubating the tubes in the tube racks in an incubator or oven.
What kind of centrifuge do I need to spin down the AutoLys M Tubes (Cat. No. A38738)?

Any centrifuge with plate adapters will work. The AutoLys M Tube Racks fit nicely into those adapters.
Do I need to buy the AutoLys M TubeLifter (Cat. No. A37956) or AutoLys M Tube Pliers (Cat. No. A38261) to use the AutoLys M Tubes (Cat. No. A38738)?

No, the AutoLys M Tubes can be lifted manually, but the tools significantly reduce hand strain and increase ease of use as well as throughput.

Do the AutoLys M Tubes come with the MagMAX FFPE DNA/RNA Ultra Kit?

No, they have to be purchased separately together with some other products that are necessary for using the AutoLys M Tube system efficiently:
- AutoLys M Tubes and Caps (Cat. No. A38738)
- Autolys M Tube Locking Lid (Cat. No. A37954)
- AutoLys M TubeLifter (Cat. No. A37956)
- AutoLys M Tube Pliers (Cat. No. A38261)
- AutoLys M Tube Racks (Cat. No. A37955)

How does using the the AutoLys M Tube system help in increase the DNA/RNA yield from FFPE samples?

The FFPE-associated wax and debris are held in the upper chamber of the AutoLys M tube while the lysate passes through. Nucleic acids can then be purified from the clarified lysate. The AutoLys M tubes thus reduce manipulation of the sample eliminating the need to pellet the tissue and wash, so tissue loss is minimized

Can I use the AutoLys M Tube system with any of your FFPE sample prep kits or has it only been optimized for the MagMAX FFPE DNA/RNA Ultra Kit?

The AutoLys M Tube system has only been tested with the MagMAX FFPE DNA/RNA Ultra Kit, but it may also work with our RecoverAll Total Nucleic Acid Isolation Kit for FFPE .

I am planning on using your MagMAX FFPE DNA/RNA Ultra Kit. I don't want to perform a deparaffinization step using organic solvents, is there an alternative reagent or method I can use?

Yes, we offer the AutoLys M Tube system, which creates cleared lysates from FFPE tissue samples without the need for deparaffinization or wash steps, while increasing DNA and RNA yields.

For Research Use Only. Not for use in diagnostic procedures.